A photoresponsive polymer surface which enables in situ control of living cell adhesion

Jun Ichi Edahiro; Kimio Sumaru; Yuichi Tada; Katsuhide Ohi; Toshiyuki Takagi; Toshio Shinbo; Toshiyuki Kanamori; Yasuo Yoshimi

A photoresponsive polymer surface which enables in situ control of living cell adhesion

Jun Ichi Edahiro, Kimio Sumaru, Yuichi Tada, Katsuhide Ohi, Toshiyuki Takagi, Toshio Shinbo, Toshiyuki Kanamori, Yasuo Yoshimi

Research output: Contribution to conference › Paper › peer-review

Abstract

Novel photoresponsive culture surface (PRCS) that can control cell adhesion by photo-irradiation and temperature change was prepared with polymethylmethacrylate and a photoresponsive polymer containing photochromic chromophore, 6-nitrospiropyran. On the PRCS, CHO-K1 cells were attached evenly after incubation at 37°C. The PRCS was regionally irradiated with light (365 nm) for 5 min and cooled in fresh medium at 15°C for 15 min. Then the PRCS was washed by fresh medium at 15°C. In the washing process, the adhering cells were immobilized in the irradiated region, while the cells were easily detached in the other region. Adhering cells were confirmed as alive because they were stained by CMTPX reagent. As a result, living cell patterning with the 100 μm line width was produced.

Original language	English
Number of pages	1
Publication status	Published - 2005 Dec 1
Event	54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: 2005 May 25 → 2005 May 27

Conference

Conference	54th SPSJ Annual Meeting 2005
Country/Territory	Japan
City	Yokohama
Period	05/5/25 → 05/5/27

Keywords

Cell adhesion control
Living cell
Photoresponsive culture surface

ASJC Scopus subject areas

Engineering(all)

Cite this

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title = "A photoresponsive polymer surface which enables in situ control of living cell adhesion",

abstract = "Novel photoresponsive culture surface (PRCS) that can control cell adhesion by photo-irradiation and temperature change was prepared with polymethylmethacrylate and a photoresponsive polymer containing photochromic chromophore, 6-nitrospiropyran. On the PRCS, CHO-K1 cells were attached evenly after incubation at 37°C. The PRCS was regionally irradiated with light (365 nm) for 5 min and cooled in fresh medium at 15°C for 15 min. Then the PRCS was washed by fresh medium at 15°C. In the washing process, the adhering cells were immobilized in the irradiated region, while the cells were easily detached in the other region. Adhering cells were confirmed as alive because they were stained by CMTPX reagent. As a result, living cell patterning with the 100 μm line width was produced.",

keywords = "Cell adhesion control, Living cell, Photoresponsive culture surface",

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T1 - A photoresponsive polymer surface which enables in situ control of living cell adhesion

AU - Edahiro, Jun Ichi

AU - Sumaru, Kimio

AU - Tada, Yuichi

AU - Ohi, Katsuhide

AU - Takagi, Toshiyuki

AU - Shinbo, Toshio

AU - Kanamori, Toshiyuki

AU - Yoshimi, Yasuo

PY - 2005/12/1

Y1 - 2005/12/1

N2 - Novel photoresponsive culture surface (PRCS) that can control cell adhesion by photo-irradiation and temperature change was prepared with polymethylmethacrylate and a photoresponsive polymer containing photochromic chromophore, 6-nitrospiropyran. On the PRCS, CHO-K1 cells were attached evenly after incubation at 37°C. The PRCS was regionally irradiated with light (365 nm) for 5 min and cooled in fresh medium at 15°C for 15 min. Then the PRCS was washed by fresh medium at 15°C. In the washing process, the adhering cells were immobilized in the irradiated region, while the cells were easily detached in the other region. Adhering cells were confirmed as alive because they were stained by CMTPX reagent. As a result, living cell patterning with the 100 μm line width was produced.

AB - Novel photoresponsive culture surface (PRCS) that can control cell adhesion by photo-irradiation and temperature change was prepared with polymethylmethacrylate and a photoresponsive polymer containing photochromic chromophore, 6-nitrospiropyran. On the PRCS, CHO-K1 cells were attached evenly after incubation at 37°C. The PRCS was regionally irradiated with light (365 nm) for 5 min and cooled in fresh medium at 15°C for 15 min. Then the PRCS was washed by fresh medium at 15°C. In the washing process, the adhering cells were immobilized in the irradiated region, while the cells were easily detached in the other region. Adhering cells were confirmed as alive because they were stained by CMTPX reagent. As a result, living cell patterning with the 100 μm line width was produced.

KW - Cell adhesion control

KW - Living cell

KW - Photoresponsive culture surface

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M3 - Paper

AN - SCOPUS:33645645369

T2 - 54th SPSJ Annual Meeting 2005

Y2 - 25 May 2005 through 27 May 2005

ER -

A photoresponsive polymer surface which enables in situ control of living cell adhesion

Abstract

Conference

Keywords

ASJC Scopus subject areas

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