Bedside cancer cell collector using size-dependent cell filtration in microfluidic chip

T. Masuda, M. Niimi, H. Nakanishi, Y. Yamanishi, F. Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have developed the microfluidic chip to isolate gastric cancer cells from the peritoneal washing. Gastric cancer cells, which tend to be agglomerated in the peritoneal washing, are bigger than the other cells and the fragment of soft tissue. In this paper, we present a novel biochip to enrich low-concentrated cancer cells in the peritoneal washing by size-dependent cell filtration. This biochip has an advantage to avoid high-accuracy control of the flow rate because the cancer cells were filtered by the gap of the microchannel. Two-step exposure was carried out to achieve the precise 3D uneven channel for cell filtration. Furthermore by setting fins in the microchannel to assist the sheath flow, the capability to enrich the cancer cells was improved. As a result, we succeeded in double enrichment of the cancer cells in the quasi peritoneal washing.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1215-1217
Number of pages3
Publication statusPublished - 2011 Dec 1
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume2

Conference

Conference15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Country/TerritoryUnited States
CitySeattle, WA
Period11/10/211/10/6

Keywords

  • Bedside
  • Cancer cell
  • Cell filtration
  • Enrichment
  • Microfluidic chip

ASJC Scopus subject areas

  • Control and Systems Engineering

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