Carcinoembryonic antigen mediates in vitro cell aggregation induced by interferon-γ in a human colon cancer cell line: requirement for active metabolism and intact cytoskeleton

Takanori Kanai; Toshifumi Hibi; Atsushi Hayashi; Junya Takashima; Masahide Shiozawa; Sadakazu Aiso; Kyoko Toda; Yasushi Iwao; Mamoru Watanabe; Masaharu Tsuchiya

doi:10.1016/0304-3835(93)90105-I

Carcinoembryonic antigen mediates in vitro cell aggregation induced by interferon-γ in a human colon cancer cell line: requirement for active metabolism and intact cytoskeleton

Takanori Kanai, Toshifumi Hibi, Atsushi Hayashi, Junya Takashima, Masahide Shiozawa, Sadakazu Aiso, Kyoko Toda, Yasushi Iwao, Mamoru Watanabe, Masaharu Tsuchiya

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The homotypic cell aggregation of a carcinoembryonic antigen (CEA) positive colon cancer cell line (Colo 205) was induced in vitro by interferon-γ (IFN-γ) treatment. Divalent cations were required for this aggregation, as it was inhibited by EDTA. The partial inhibition by cytochalasin B and the complete inhibition by a mixture of sodium azide and 2-deoxyglucose suggests that the aggregation requires the integrity of cytoskeleton and active metabolism. The expression of CEA was increased in the cytoplasm and on the membrane of Colo 205 by IFN-γ treatment. Furthermore, this aggregation was inhibited completely by anti-CEA monoclonal antibody (mAb) and partially by mAb against intercellular adhesion molecule-1. This in vitro study suggests that CEA molecule participates in the IFN-γ induced homotypic adhesion of some CEA positive cancer cells and that IFN-γ has an important role in the regulation of cell-cell interaction mediated by CEA molecule.

Original language	English
Pages (from-to)	109-117
Number of pages	9
Journal	Cancer Letters
Volume	71
Issue number	1-3
DOIs	https://doi.org/10.1016/0304-3835(93)90105-I
Publication status	Published - 1993 Jul 30
Externally published	Yes

Keywords

carcinoembryonic antigen
cell adhesion
cell aggregation
colon cancer
interferon-γ

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1016/0304-3835(93)90105-I

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Kanai, T., Hibi, T., Hayashi, A., Takashima, J., Shiozawa, M., Aiso, S., Toda, K., Iwao, Y., Watanabe, M., & Tsuchiya, M. (1993). Carcinoembryonic antigen mediates in vitro cell aggregation induced by interferon-γ in a human colon cancer cell line: requirement for active metabolism and intact cytoskeleton. Cancer Letters, 71(1-3), 109-117. https://doi.org/10.1016/0304-3835(93)90105-I

Kanai, T, Hibi, T, Hayashi, A, Takashima, J, Shiozawa, M, Aiso, S, Toda, K, Iwao, Y, Watanabe, M & Tsuchiya, M 1993, 'Carcinoembryonic antigen mediates in vitro cell aggregation induced by interferon-γ in a human colon cancer cell line: requirement for active metabolism and intact cytoskeleton', Cancer Letters, vol. 71, no. 1-3, pp. 109-117. https://doi.org/10.1016/0304-3835(93)90105-I

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title = "Carcinoembryonic antigen mediates in vitro cell aggregation induced by interferon-γ in a human colon cancer cell line: requirement for active metabolism and intact cytoskeleton",

abstract = "The homotypic cell aggregation of a carcinoembryonic antigen (CEA) positive colon cancer cell line (Colo 205) was induced in vitro by interferon-γ (IFN-γ) treatment. Divalent cations were required for this aggregation, as it was inhibited by EDTA. The partial inhibition by cytochalasin B and the complete inhibition by a mixture of sodium azide and 2-deoxyglucose suggests that the aggregation requires the integrity of cytoskeleton and active metabolism. The expression of CEA was increased in the cytoplasm and on the membrane of Colo 205 by IFN-γ treatment. Furthermore, this aggregation was inhibited completely by anti-CEA monoclonal antibody (mAb) and partially by mAb against intercellular adhesion molecule-1. This in vitro study suggests that CEA molecule participates in the IFN-γ induced homotypic adhesion of some CEA positive cancer cells and that IFN-γ has an important role in the regulation of cell-cell interaction mediated by CEA molecule.",

keywords = "carcinoembryonic antigen, cell adhesion, cell aggregation, colon cancer, interferon-γ",

author = "Takanori Kanai and Toshifumi Hibi and Atsushi Hayashi and Junya Takashima and Masahide Shiozawa and Sadakazu Aiso and Kyoko Toda and Yasushi Iwao and Mamoru Watanabe and Masaharu Tsuchiya",

note = "Funding Information: This work was supported in part by a Grant-in-Aid from the Ministry of Health and Welfare of Japan.",

year = "1993",

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doi = "10.1016/0304-3835(93)90105-I",

language = "English",

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T1 - Carcinoembryonic antigen mediates in vitro cell aggregation induced by interferon-γ in a human colon cancer cell line

T2 - requirement for active metabolism and intact cytoskeleton

AU - Kanai, Takanori

AU - Hibi, Toshifumi

AU - Hayashi, Atsushi

AU - Takashima, Junya

AU - Shiozawa, Masahide

AU - Aiso, Sadakazu

AU - Toda, Kyoko

AU - Iwao, Yasushi

AU - Watanabe, Mamoru

AU - Tsuchiya, Masaharu

N1 - Funding Information: This work was supported in part by a Grant-in-Aid from the Ministry of Health and Welfare of Japan.

PY - 1993/7/30

Y1 - 1993/7/30

N2 - The homotypic cell aggregation of a carcinoembryonic antigen (CEA) positive colon cancer cell line (Colo 205) was induced in vitro by interferon-γ (IFN-γ) treatment. Divalent cations were required for this aggregation, as it was inhibited by EDTA. The partial inhibition by cytochalasin B and the complete inhibition by a mixture of sodium azide and 2-deoxyglucose suggests that the aggregation requires the integrity of cytoskeleton and active metabolism. The expression of CEA was increased in the cytoplasm and on the membrane of Colo 205 by IFN-γ treatment. Furthermore, this aggregation was inhibited completely by anti-CEA monoclonal antibody (mAb) and partially by mAb against intercellular adhesion molecule-1. This in vitro study suggests that CEA molecule participates in the IFN-γ induced homotypic adhesion of some CEA positive cancer cells and that IFN-γ has an important role in the regulation of cell-cell interaction mediated by CEA molecule.

AB - The homotypic cell aggregation of a carcinoembryonic antigen (CEA) positive colon cancer cell line (Colo 205) was induced in vitro by interferon-γ (IFN-γ) treatment. Divalent cations were required for this aggregation, as it was inhibited by EDTA. The partial inhibition by cytochalasin B and the complete inhibition by a mixture of sodium azide and 2-deoxyglucose suggests that the aggregation requires the integrity of cytoskeleton and active metabolism. The expression of CEA was increased in the cytoplasm and on the membrane of Colo 205 by IFN-γ treatment. Furthermore, this aggregation was inhibited completely by anti-CEA monoclonal antibody (mAb) and partially by mAb against intercellular adhesion molecule-1. This in vitro study suggests that CEA molecule participates in the IFN-γ induced homotypic adhesion of some CEA positive cancer cells and that IFN-γ has an important role in the regulation of cell-cell interaction mediated by CEA molecule.

KW - carcinoembryonic antigen

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KW - cell aggregation

KW - colon cancer

KW - interferon-γ

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Carcinoembryonic antigen mediates in vitro cell aggregation induced by interferon-γ in a human colon cancer cell line: requirement for active metabolism and intact cytoskeleton

Abstract

Keywords

ASJC Scopus subject areas

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