Chemiluminescent imaging of transpired ethanol from the palm for evaluation of alcohol metabolism

Takahiro Arakawa, Kazutaka Kita, Xin Wang, Kumiko Miyajima, Koji Toma, Kohji Mitsubayashi

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)


A 2-dimensional imaging system was constructed and applied in measurements of gaseous ethanol emissions from the human palm. This imaging system measures gaseous ethanol concentrations as intensities of chemiluminescence by luminol reaction induced by alcohol oxidase and luminol-hydrogen peroxide-horseradish peroxidase system. Conversions of ethanol distributions and concentrations to 2-dimensional chemiluminescence were conducted on an enzyme-immobilized mesh substrate in a dark box, which contained a luminol solution. In order to visualize ethanol emissions from human palm skin, we developed highly sensitive and selective imaging system for transpired gaseous ethanol at sub ppm-levels. Thus, a mixture of a high-purity luminol solution of luminol sodium salt HG solution instead of standard luminol solution and an enhancer of eosin Y solution was adapted to refine the chemiluminescent intensity of the imaging system, and improved the detection limit to 3. ppm gaseous ethanol. The highly sensitive imaging allows us to successfully visualize the emissions dynamics of transdermal gaseous ethanol. The intensity of each site on the palm shows the reflection of ethanol concentrations distributions corresponding to the amount of alcohol metabolized upon consumption. This imaging system is significant and useful for the assessment of ethanol measurement of the palmar skin.

Original languageEnglish
Pages (from-to)570-575
Number of pages6
JournalBiosensors and Bioelectronics
Publication statusPublished - 2015 May 5
Externally publishedYes


  • Chemiluminescence
  • Gaseous ethanol
  • Imaging system
  • Oral alcohol administration
  • Transdermal emissions

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry


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