Cloning and expression of Xenopus laevis xSox12 cDNA

Naoki Komatsu; Yoshiki Hiraoka; Masahide Shiozawa; Motoyuki Ogawa; Sadakazu Aiso

doi:10.1016/0167-4781(95)00228-6

Cloning and expression of Xenopus laevis xSox12 cDNA

Naoki Komatsu, Yoshiki Hiraoka, Masahide Shiozawa, Motoyuki Ogawa, Sadakazu Aiso

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding xSox12 from Xenopus laevis ovary. The cDNA contained an open reading frame (ORF) coding for 470 amino acids encompassing an HMG box characteristic of the SOX family, a leucine zipper motif and glutamine-rich segments. The size of the xSox12 mRNA was determined to be 3.0 knt by Northern analysis. The ovary was the most prominent in the expression of the Sox mRNA among the various tissues of adult frog as far as examined.

Original language	English
Pages (from-to)	117-119
Number of pages	3
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1305
Issue number	3
DOIs	https://doi.org/10.1016/0167-4781(95)00228-6
Publication status	Published - 1996 Mar 1
Externally published	Yes

Keywords

Molecular cloning
Nucleotide sequence
SOX
Xenopus laevis
cDNA

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/0167-4781(95)00228-6

Cite this

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title = "Cloning and expression of Xenopus laevis xSox12 cDNA",

abstract = "A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding xSox12 from Xenopus laevis ovary. The cDNA contained an open reading frame (ORF) coding for 470 amino acids encompassing an HMG box characteristic of the SOX family, a leucine zipper motif and glutamine-rich segments. The size of the xSox12 mRNA was determined to be 3.0 knt by Northern analysis. The ovary was the most prominent in the expression of the Sox mRNA among the various tissues of adult frog as far as examined.",

keywords = "Molecular cloning, Nucleotide sequence, SOX, Xenopus laevis, cDNA",

author = "Naoki Komatsu and Yoshiki Hiraoka and Masahide Shiozawa and Motoyuki Ogawa and Sadakazu Aiso",

note = "Funding Information: This work was supported in part by a grant from Ministry of Education, Science and Culture to S.A. and by Keio Gakuji Fukuzawa Memorial Fund for the Advancement of Education and Research from Keio University to Y.H.",

year = "1996",

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doi = "10.1016/0167-4781(95)00228-6",

language = "English",

volume = "1305",

pages = "117--119",

journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",

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TY - JOUR

T1 - Cloning and expression of Xenopus laevis xSox12 cDNA

AU - Komatsu, Naoki

AU - Hiraoka, Yoshiki

AU - Shiozawa, Masahide

AU - Ogawa, Motoyuki

AU - Aiso, Sadakazu

N1 - Funding Information: This work was supported in part by a grant from Ministry of Education, Science and Culture to S.A. and by Keio Gakuji Fukuzawa Memorial Fund for the Advancement of Education and Research from Keio University to Y.H.

PY - 1996/3/1

Y1 - 1996/3/1

N2 - A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding xSox12 from Xenopus laevis ovary. The cDNA contained an open reading frame (ORF) coding for 470 amino acids encompassing an HMG box characteristic of the SOX family, a leucine zipper motif and glutamine-rich segments. The size of the xSox12 mRNA was determined to be 3.0 knt by Northern analysis. The ovary was the most prominent in the expression of the Sox mRNA among the various tissues of adult frog as far as examined.

AB - A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding xSox12 from Xenopus laevis ovary. The cDNA contained an open reading frame (ORF) coding for 470 amino acids encompassing an HMG box characteristic of the SOX family, a leucine zipper motif and glutamine-rich segments. The size of the xSox12 mRNA was determined to be 3.0 knt by Northern analysis. The ovary was the most prominent in the expression of the Sox mRNA among the various tissues of adult frog as far as examined.

KW - Molecular cloning

KW - Nucleotide sequence

KW - SOX

KW - Xenopus laevis

KW - cDNA

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AN - SCOPUS:0030031017

SN - 0167-4781

VL - 1305

SP - 117

EP - 119

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

IS - 3

ER -

Cloning and expression of Xenopus laevis xSox12 cDNA

Abstract

Keywords

ASJC Scopus subject areas

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