Computer-controlled microcirculatory support system for endothelial cell culture and shearing

Jonathan W. Song, Wei Gu, Nobuyuki Futai, Kristy A. Warner, Jacques E. Nor, Shuichi Takayama

Research output: Contribution to journalArticlepeer-review

216 Citations (Scopus)


Endothelial cells (ECs) lining the inner lumen of blood vessels are continuously subjected to hemodynamic shear stress, which is known to modify EC morphology and biological activity. This paper describes a self-contained microcirculatory EC culture system that efficiently studies such effects of shear stress on EC alignment and elongation in vitro. The culture system is composed of elastomeric microfluidic cell shearing chambers interfaced with computer-controlled movement of piezoelectric pins on a refreshable Braille display. The flow rate is varied by design of channels that allow for movement of different volumes of fluid per variable-speed pump stroke. The integrated microfluidic valving and pumping system allowed primary EC seeding and differential shearing in multiple compartments to be performed on a single chip. The microfluidic flows caused ECs to align and elongate significantly in the direction of flow according to their exposed levels of shear stress. This microfluidic system overcomes the small flow rates and the inefficiencies of previously described microfluidic and macroscopic systems respectively to conveniently perform parallel studies of EC response to shear stress.

Original languageEnglish
Pages (from-to)3993-3999
Number of pages7
JournalAnalytical Chemistry
Issue number13
Publication statusPublished - 2005 Jul 1
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry


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