Construction of DNA-Au nanoparticles multilayer and its application to detection of DNA hybridization

Hongchun Liu, Akira Yamaguchi, Masayuki Hayashida, Shigeki Matsuo, Hiroaki Misawa

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


We have developed a sensitive detection system for DNA hybridization containing DNA-Au nanoparticles (DNA-GPs) multilayer which leads to the enhancement of the detection sensitivity of surface plasmon resonance (SPR) signals. The SPR angular shift, which is the difference in the SPR angles before and after hybridization, becomes amplified with increasing number of layers of the DNA-GPs. A similar increase in SPR angular shift was obtained for the target DNA with low concentration. The reason for the enhancement is attributed to the interaction between Au nanoparticles and the Au film, as well as the increase in immobilized probe DNA content on the substrate.

Original languageEnglish
Pages (from-to)2767-2770
Number of pages4
JournalJapanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
Issue number5 A
Publication statusPublished - 2004 May
Externally publishedYes


  • Au nanoparticles
  • DNA
  • Hybridization
  • Multilayer
  • Surface plasmon resonance (SPR)

ASJC Scopus subject areas

  • General Engineering
  • General Physics and Astronomy


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