Contribution of blood volume changes to intrinsic optical signals

Fukuda Mitsuhiro, Rajagopalan Uma Maheswari, Homma Ryota, Tanifuji Manabu

Research output: Contribution to journalArticlepeer-review


Hemodynamic responses evoked by neural activation are used to visualize active brain areas in various functional brain imaging techniques, such as PET, fMRI and intrinsic signal imaging. Though the major focus of these techniques is spatial localization of the active areas, the spatial precision of hemodynamic responses is not well understood. The purpose of this study was to investigate the spatial precision of hemodynamic responses by using intrinsic signal imaging. In particular, we focused on activity-dependent changes in the blood volume. We visualized sub-millimeter-scale functional structures such as orientation columns in the feline visual cortex using intrinsic signal imaging. To investigate the spatial precision of changes in the blood volume, we extracted the blood volume component from intrinsic signals using the Beer–Lambert equation, and directly visualized changes in the blood volume by observing absorption changes of an extrinsic dye infused into the bloodstream. In both cases, we succeeded in visualizing sub-millimeter-scale functional structures as changes in the blood volume. Taking into account that spatial separation of arteries is coarser than the spatial layout of sub-millimeter-scale functional structures, we believe these results suggest that there are supplementary control mechanisms of blood flow, probably in the capillary beds, in addition to arterial global control.

Original languageEnglish
Pages (from-to)165-171
Number of pages7
JournalInternational Congress Series
Issue numberC
Publication statusPublished - 2002 Jul 1
Externally publishedYes


  • Beer–Lambert law
  • Capillary
  • Hemodynamic responses
  • Orientation column
  • Visual cortex

ASJC Scopus subject areas

  • Medicine(all)


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