Cultivation characteristics of denitrification by thermophilic Geobacillus sp. strain TDN01

Masatomo Mishima, Kenichi Iwata, Kota Nara, Toru Matsui, Toshiya Shigeno, Toshio Omori

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


Nine thermophilic denitrification bacteria were isolated from field soil, mud, and spa samples. The alignment of 16S rDNA showed that all were identical to the genus Geobacillus. Two of the bacteria produced N2O and N2 gas and the other seven strains produced N2 gas from nitrate. We examined the growth substrates for Geobacillus TDN01 and determined that sodium succinate, pyruvate, formate, acetate, glycerol, glucose, sucrose, and cellobiose well supported growth of the isolate. Growth occurred under the following concentration of NO3- and phosphate: 10-60 mmol/L, and 0.1-50 mmol/L, respectively. Thermophilic TDN01 grown on sodium succinate accumulated nitrite. A time course of denitrification by Geobacillus TDN01 in a jar fermentor revealed that maintaining a pH of around 7 is important for denitrification without accumulating NO2. The NO3- and NO2- consumption ratios of Geobacillus were 44-75 and 9-41 times higher, respectively, than those of Pseudomonas stutzeri JCM 5965T.

Original languageEnglish
Pages (from-to)81-86
Number of pages6
JournalJournal of General and Applied Microbiology
Issue number2
Publication statusPublished - 2009


  • Denitrification
  • Geobacillus
  • Nitrate
  • Nitrite
  • Thermophilic bacteria

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology


Dive into the research topics of 'Cultivation characteristics of denitrification by thermophilic Geobacillus sp. strain TDN01'. Together they form a unique fingerprint.

Cite this