TY - JOUR
T1 - Degradation of chitosan hydrogel dispersed in dilute carboxylic acids by solution plasma and evaluation of anticancer activity of degraded products
AU - Chokradjaroen, Chayanaphat
AU - Rujiravanit, Ratana
AU - Theeramunkong, Sewan
AU - Saito, Nagahiro
N1 - Funding Information:
CC would like to express thank to the Thailand Research Fund (TRF) for financial support through a Royal Golden Jubilee Ph.D. program (RGJ-Ph.D.: Grant Number PHD= 0179=2556). This work was financially supported by the Thailand Research Fund (TRF) under contract number BRG5480008, the National Research Funding from the Government of Thailand given to Chulalongkorn University, and Japan Science and Technology Agency: Core Research for Evolutional Science and Technology (JST=CREST: Grant Number GJPMJCR12L1). Moreover, the authors would like to acknowlege Surapon Foods Public Co., Ltd. (Thailand) for providing the shrimp shells and Nagoya University (Japan) for providing solution plasma equipment. In addition, the authors would like to thank the Drug Discovery and Development Center at Thammasat University (Thailand) for providing all facilities that were used for the biological tests.
Publisher Copyright:
© 2018 The Japan Society of Applied Physics.
PY - 2018/1
Y1 - 2018/1
N2 - Chitosan is a polysaccharide that has been extensively studied in the field of biomedicine, especially its water-soluble degraded products called chitooligosaccharides (COS). In this study, COS were produced by the degradation of chitosan hydrogel dispersed in a dilute solution (i.e., 1.55 mM) of various kinds of carboxylic acids using a non-thermal plasma technology called solution plasma (SP). The degradation rates of chitosan were influenced by the type of carboxylic acids, depending on the interaction between chitosan and each carboxylic acid. After SP treatment, the water-soluble degraded products containing COS could be easily separated from the water-insoluble residue of chitosan hydrogel by centrifugation. The production yields of the COS were mostly higher than 55%. Furthermore, the obtained COS products were evaluated for their inhibitory effect as well as their selectivity against human lung cancer cells (H460) and human lung normal cells (MRC-5).
AB - Chitosan is a polysaccharide that has been extensively studied in the field of biomedicine, especially its water-soluble degraded products called chitooligosaccharides (COS). In this study, COS were produced by the degradation of chitosan hydrogel dispersed in a dilute solution (i.e., 1.55 mM) of various kinds of carboxylic acids using a non-thermal plasma technology called solution plasma (SP). The degradation rates of chitosan were influenced by the type of carboxylic acids, depending on the interaction between chitosan and each carboxylic acid. After SP treatment, the water-soluble degraded products containing COS could be easily separated from the water-insoluble residue of chitosan hydrogel by centrifugation. The production yields of the COS were mostly higher than 55%. Furthermore, the obtained COS products were evaluated for their inhibitory effect as well as their selectivity against human lung cancer cells (H460) and human lung normal cells (MRC-5).
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U2 - 10.7567/JJAP.57.0102B5
DO - 10.7567/JJAP.57.0102B5
M3 - Article
AN - SCOPUS:85040318014
SN - 0021-4922
VL - 57
JO - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes
JF - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes
IS - 1
M1 - 0102B5
ER -