Detection of the red tide-causing plankton Alexandrium affine by a piezoelectric immunosensor using a novel method of immobilizing antibodies

Keijiro Nakanishi; Adachi Masao; Yoshihiko Sako; Yuzaburo Ishida; Hitoshi Muguruma; Isao Karube

doi:10.1080/00032719608001478

Detection of the red tide-causing plankton Alexandrium affine by a piezoelectric immunosensor using a novel method of immobilizing antibodies

Keijiro Nakanishi, Adachi Masao, Yoshihiko Sako, Yuzaburo Ishida, Hitoshi Muguruma, Isao Karube

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

In this paper, the determination of Alexandrium affine in sea water was performed using piezoelectric immunosensors. In addition, we report a novel method of immobilizing antibodies on the quartz crystal immunosensors using plasma-polymerized ethylenediamine film. The films formed on the quartz crystals are extremely thin, homogeneous and incorporate amino groups. Sensors produced using this method show higher sensitivity than sensors made using conventional immobilization methods for example polyethylenimine or γ-aminopropyltrimethoxysilane.

Original language	English
Pages (from-to)	1247-1258
Number of pages	12
Journal	Analytical Letters
Volume	29
Issue number	8
DOIs	https://doi.org/10.1080/00032719608001478
Publication status	Published - 1996
Externally published	Yes

Keywords

Alexandrium affine
IgM
Monoclonal antibody
Piezoelectric crystal
Plasma-polymerized Films

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Spectroscopy
Clinical Biochemistry
Biochemistry, medical
Electrochemistry

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10.1080/00032719608001478

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title = "Detection of the red tide-causing plankton Alexandrium affine by a piezoelectric immunosensor using a novel method of immobilizing antibodies",

abstract = "In this paper, the determination of Alexandrium affine in sea water was performed using piezoelectric immunosensors. In addition, we report a novel method of immobilizing antibodies on the quartz crystal immunosensors using plasma-polymerized ethylenediamine film. The films formed on the quartz crystals are extremely thin, homogeneous and incorporate amino groups. Sensors produced using this method show higher sensitivity than sensors made using conventional immobilization methods for example polyethylenimine or γ-aminopropyltrimethoxysilane.",

keywords = "Alexandrium affine, IgM, Monoclonal antibody, Piezoelectric crystal, Plasma-polymerized Films",

author = "Keijiro Nakanishi and Adachi Masao and Yoshihiko Sako and Yuzaburo Ishida and Hitoshi Muguruma and Isao Karube",

year = "1996",

doi = "10.1080/00032719608001478",

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T1 - Detection of the red tide-causing plankton Alexandrium affine by a piezoelectric immunosensor using a novel method of immobilizing antibodies

AU - Nakanishi, Keijiro

AU - Masao, Adachi

AU - Sako, Yoshihiko

AU - Ishida, Yuzaburo

AU - Muguruma, Hitoshi

AU - Karube, Isao

PY - 1996

Y1 - 1996

N2 - In this paper, the determination of Alexandrium affine in sea water was performed using piezoelectric immunosensors. In addition, we report a novel method of immobilizing antibodies on the quartz crystal immunosensors using plasma-polymerized ethylenediamine film. The films formed on the quartz crystals are extremely thin, homogeneous and incorporate amino groups. Sensors produced using this method show higher sensitivity than sensors made using conventional immobilization methods for example polyethylenimine or γ-aminopropyltrimethoxysilane.

AB - In this paper, the determination of Alexandrium affine in sea water was performed using piezoelectric immunosensors. In addition, we report a novel method of immobilizing antibodies on the quartz crystal immunosensors using plasma-polymerized ethylenediamine film. The films formed on the quartz crystals are extremely thin, homogeneous and incorporate amino groups. Sensors produced using this method show higher sensitivity than sensors made using conventional immobilization methods for example polyethylenimine or γ-aminopropyltrimethoxysilane.

KW - Alexandrium affine

KW - IgM

KW - Monoclonal antibody

KW - Piezoelectric crystal

KW - Plasma-polymerized Films

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JO - Analytical Letters

JF - Analytical Letters

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ER -

Detection of the red tide-causing plankton Alexandrium affine by a piezoelectric immunosensor using a novel method of immobilizing antibodies

Abstract

Keywords

ASJC Scopus subject areas

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