Determination of lactate by sequential injection analysis using a fluoride ion-selective electrode detector

Yuichi Takahashi; Takashi Masadome

doi:10.5796/ELECTROCHEMISTRY.20-00097

Determination of lactate by sequential injection analysis using a fluoride ion-selective electrode detector

Yuichi Takahashi, Takashi Masadome

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

A combination of sequential Injection Analysis (SIA) system and the ion-selective electrode (ISE) detector is considered to be one of a very effective analytical method. Reaction of lactate with lactate oxidase produces pyruvic acid and hydrogen peroxide. When the produced hydrogen peroxide is reacted with 4-aminoantipyrine (4-AAP) and 4-fluorophenol (4-FP) in the presence of horseradish peroxidase (HRP), F⁻ ion is produced. The concentration of lactate can be indirectly determined by measuring the F⁻ ion by F⁻ ISE. Based on this principle of detection of lactate, a new measurement method of lactate using an SIA with ISE detector was developed.

Original language	English
Pages (from-to)	522-524
Number of pages	3
Journal	Electrochemistry
Volume	88
Issue number	6
DOIs	https://doi.org/10.5796/ELECTROCHEMISTRY.20-00097
Publication status	Published - 2020 Nov 5

Keywords

Ion-selective Electrode
Lactate
Sequential Injection Analysis

ASJC Scopus subject areas

Electrochemistry

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10.5796/ELECTROCHEMISTRY.20-00097

Cite this

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title = "Determination of lactate by sequential injection analysis using a fluoride ion-selective electrode detector",

abstract = "A combination of sequential Injection Analysis (SIA) system and the ion-selective electrode (ISE) detector is considered to be one of a very effective analytical method. Reaction of lactate with lactate oxidase produces pyruvic acid and hydrogen peroxide. When the produced hydrogen peroxide is reacted with 4-aminoantipyrine (4-AAP) and 4-fluorophenol (4-FP) in the presence of horseradish peroxidase (HRP), F− ion is produced. The concentration of lactate can be indirectly determined by measuring the F− ion by F− ISE. Based on this principle of detection of lactate, a new measurement method of lactate using an SIA with ISE detector was developed.",

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T1 - Determination of lactate by sequential injection analysis using a fluoride ion-selective electrode detector

AU - Takahashi, Yuichi

AU - Masadome, Takashi

N1 - Publisher Copyright: © The Author(s) 2020. Published by ECSJ. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 License

PY - 2020/11/5

Y1 - 2020/11/5

N2 - A combination of sequential Injection Analysis (SIA) system and the ion-selective electrode (ISE) detector is considered to be one of a very effective analytical method. Reaction of lactate with lactate oxidase produces pyruvic acid and hydrogen peroxide. When the produced hydrogen peroxide is reacted with 4-aminoantipyrine (4-AAP) and 4-fluorophenol (4-FP) in the presence of horseradish peroxidase (HRP), F− ion is produced. The concentration of lactate can be indirectly determined by measuring the F− ion by F− ISE. Based on this principle of detection of lactate, a new measurement method of lactate using an SIA with ISE detector was developed.

AB - A combination of sequential Injection Analysis (SIA) system and the ion-selective electrode (ISE) detector is considered to be one of a very effective analytical method. Reaction of lactate with lactate oxidase produces pyruvic acid and hydrogen peroxide. When the produced hydrogen peroxide is reacted with 4-aminoantipyrine (4-AAP) and 4-fluorophenol (4-FP) in the presence of horseradish peroxidase (HRP), F− ion is produced. The concentration of lactate can be indirectly determined by measuring the F− ion by F− ISE. Based on this principle of detection of lactate, a new measurement method of lactate using an SIA with ISE detector was developed.

KW - Ion-selective Electrode

KW - Lactate

KW - Sequential Injection Analysis

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Determination of lactate by sequential injection analysis using a fluoride ion-selective electrode detector

Abstract

Keywords

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