Abstract
Using a functional polymer containing nitrospirobenzopyran residues and poly(ethylene glycol), we developed a photoresponsive cell culture surface (PRCS), at which the cell adhesion can be enhanced locally by UV light irradiation (350-400 nm, 35 mW/cm2, 5 min) even on the region where living cells exist. Cell adhesion was evaluated by observing BALB/3T3 fibroblasts, which had been seeded on PRCS and then irradiated with UV light regionally, after 12 h incubation and subsequent washing to remove scarcely adhering cells. As a result, it was confirmed that the number of remaining cells in irradiated region was at most 2.5 times greater than that in nonirradiated regions, suggesting the implementation of a novel scheme to manipulate living cells individually by light irradiation in a parallel and simultaneous manner. Influence of the composition of the polymer material consisting the substrate surface was also investigated systematically.
| Original language | English |
|---|---|
| Pages (from-to) | 495-499 |
| Number of pages | 5 |
| Journal | Journal of Applied Polymer Science |
| Volume | 100 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2006 Apr 5 |
Keywords
- Cell adhesion
- Cell culture
- Light irradiation
- Nitrospirobenzopyran
- Photo-response
ASJC Scopus subject areas
- Chemistry(all)
- Surfaces, Coatings and Films
- Polymers and Plastics
- Materials Chemistry