Development of "brain-on-chip" devise including micropump for rapid administration of transmitters by and voltage imaging system for neurosignal detection

K. Harada, Y. Yoshimi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We developed a microchip for kinetic analysis of neural response toward chemical stimulation by using rapid electrochemical micropump and realtime voltage imaging. Aplysia neurons stained with a voltage sensitive dye was stimulated by a solution of potassium chloride or acetylcholine administrated by the diaphragm micropump powered by electrolysis. The change in the membrane potential of the neuron was monitored by a fluorescent microscope. The neural response time to acetylcholine was twice as long as that to potassium chloride. The device may be useful for observation of the kinetic effect of therapeutic drugs on neural response to transmitters.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages605-606
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 2016 Oct 92016 Oct 13

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/TerritoryIreland
CityDublin
Period16/10/916/10/13

Keywords

  • Brain-on-chip
  • Neural signal
  • Polydimethylsiloxane
  • Voltage sensitive dye imaging

ASJC Scopus subject areas

  • Control and Systems Engineering

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