Fast electron transfer between glucose oxidase and electrodes via phenothiazine mediators with poly(ethylene oxide) spacers attached to the enzyme surface

Kazumichi Ban; Takeshi Ueki; Yoshinori Tamada; Takahiro Saito; Shin ichiro Imabayashi; Masayoshi Watanabe

doi:10.1016/S1388-2481(01)00236-3

Fast electron transfer between glucose oxidase and electrodes via phenothiazine mediators with poly(ethylene oxide) spacers attached to the enzyme surface

Kazumichi Ban, Takeshi Ueki, Yoshinori Tamada, Takahiro Saito, Shin ichiro Imabayashi, Masayoshi Watanabe

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

A series of glucose oxidase hybrids capable of a direct electrical communication with electrodes is synthesized by covalently bonding phenothiazine mediators to surface lysine residues via poly(ethylene oxide) spacers with different lengths. The hybrid with the optimum length of spacer chain exhibits the fast electron transfer between the redox center of enzyme and electrodes. This fast electron transfer in the hybrids realizes a large catalytic current comparable to that for the corresponding freely diffusing mediator systems.

Original language	English
Pages (from-to)	649-653
Number of pages	5
Journal	Electrochemistry Communications
Volume	3
Issue number	11
DOIs	https://doi.org/10.1016/S1388-2481(01)00236-3
Publication status	Published - 2001
Externally published	Yes

Keywords

Bioconjugates
Electron transfer
Glucose oxidase
Phenothiazine
Poly(ethylene oxide)

ASJC Scopus subject areas

Electrochemistry

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10.1016/S1388-2481(01)00236-3

Cite this

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title = "Fast electron transfer between glucose oxidase and electrodes via phenothiazine mediators with poly(ethylene oxide) spacers attached to the enzyme surface",

abstract = "A series of glucose oxidase hybrids capable of a direct electrical communication with electrodes is synthesized by covalently bonding phenothiazine mediators to surface lysine residues via poly(ethylene oxide) spacers with different lengths. The hybrid with the optimum length of spacer chain exhibits the fast electron transfer between the redox center of enzyme and electrodes. This fast electron transfer in the hybrids realizes a large catalytic current comparable to that for the corresponding freely diffusing mediator systems.",

keywords = "Bioconjugates, Electron transfer, Glucose oxidase, Phenothiazine, Poly(ethylene oxide)",

author = "Kazumichi Ban and Takeshi Ueki and Yoshinori Tamada and Takahiro Saito and Imabayashi, {Shin ichiro} and Masayoshi Watanabe",

note = "Funding Information: This work was partly supported by Grant-in-Aid for Scientific Research on Priority Areas of “Molecular Synchronization for Design of New Materials System” (No. 404/11167234 for M.W.) from the Ministry of Education, Culture, Sports, Science, and Technology, Japan.",

year = "2001",

doi = "10.1016/S1388-2481(01)00236-3",

language = "English",

volume = "3",

pages = "649--653",

journal = "Electrochemistry Communications",

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publisher = "Elsevier Inc.",

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TY - JOUR

T1 - Fast electron transfer between glucose oxidase and electrodes via phenothiazine mediators with poly(ethylene oxide) spacers attached to the enzyme surface

AU - Ban, Kazumichi

AU - Ueki, Takeshi

AU - Tamada, Yoshinori

AU - Saito, Takahiro

AU - Imabayashi, Shin ichiro

AU - Watanabe, Masayoshi

N1 - Funding Information: This work was partly supported by Grant-in-Aid for Scientific Research on Priority Areas of “Molecular Synchronization for Design of New Materials System” (No. 404/11167234 for M.W.) from the Ministry of Education, Culture, Sports, Science, and Technology, Japan.

PY - 2001

Y1 - 2001

N2 - A series of glucose oxidase hybrids capable of a direct electrical communication with electrodes is synthesized by covalently bonding phenothiazine mediators to surface lysine residues via poly(ethylene oxide) spacers with different lengths. The hybrid with the optimum length of spacer chain exhibits the fast electron transfer between the redox center of enzyme and electrodes. This fast electron transfer in the hybrids realizes a large catalytic current comparable to that for the corresponding freely diffusing mediator systems.

AB - A series of glucose oxidase hybrids capable of a direct electrical communication with electrodes is synthesized by covalently bonding phenothiazine mediators to surface lysine residues via poly(ethylene oxide) spacers with different lengths. The hybrid with the optimum length of spacer chain exhibits the fast electron transfer between the redox center of enzyme and electrodes. This fast electron transfer in the hybrids realizes a large catalytic current comparable to that for the corresponding freely diffusing mediator systems.

KW - Bioconjugates

KW - Electron transfer

KW - Glucose oxidase

KW - Phenothiazine

KW - Poly(ethylene oxide)

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DO - 10.1016/S1388-2481(01)00236-3

M3 - Article

AN - SCOPUS:0034753671

SN - 1388-2481

VL - 3

SP - 649

EP - 653

JO - Electrochemistry Communications

JF - Electrochemistry Communications

IS - 11

ER -

Fast electron transfer between glucose oxidase and electrodes via phenothiazine mediators with poly(ethylene oxide) spacers attached to the enzyme surface

Abstract

Keywords

ASJC Scopus subject areas

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