Feasibility evaluation of 3D photoacoustic imaging of blood vessel structure using multiple wavelengths with a handheld probe

Yo Uchimoto, Takeshi Namita, Kengo Kondo, Makoto Yamakawa, Tsuyoshi Shiina

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)


Photoacoustic imaging is anticipated for use in portraying blood vessel structures (e.g. neovascularization in inflamed regions). To reduce invasiveness and enhance ease handling, we developed a handheld photoacoustic imaging system using multiple wavelengths. The usefulness of the proposed system was investigated in phantom experiments and in vivo measurements. A silicon tube was embedded into chicken breast meat to simulate the blood vessel. The tube was filled with ovine blood. Then laser light was guided to the phantom surface by an optical fiber bundle close to the linear ultrasound probe. Photoacoustic images were obtained at 750-950 nm wavelengths. Strong photoacoustic signals from the boundary between blood and silicon tube are observed in these images. The shape of photoacoustic spectrum at the boundary resembles that of the HbO2 absorption spectrum at 750-920 nm. In photoacoustic images, similarity between photoacoustic spectrum and HbO2 absorption spectrum was evaluated by calculating the normalized correlation coefficient. Results show high correlation in regions of strong photoacoustic signals in photoacoustic images. These analyses demonstrate the feasibility of portraying blood vessel structures under practical conditions. To evaluate the feasibility of three-dimensional vascular imaging, in vivo experiments were conducted using three wavelengths. A right hand and ultrasound probe were set in degassed water. By scanning a probe, cross-sectional ultrasound and photoacoustic images were obtained at each location. Then, all ultrasound or photoacoustic images were piled up respectively. Then three-dimensional images were constructed. Resultant images portrayed blood vessel-like structures three-dimensionally. Furthermore, to distinguish blood vessels from other tissues (e.g. skin), distinguishing images of them were constructed by comparing photoacoustic signal intensity among three wavelengths. The resultant image portrayed blood vessels as distinguished from surrounding tissues. These results demonstrated the usefulness of the proposed imaging device.

Original languageEnglish
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2018
EditorsLihong V. Wang, Alexander A. Oraevsky
ISBN (Electronic)9781510614734
Publication statusPublished - 2018
Externally publishedYes
EventPhotons Plus Ultrasound: Imaging and Sensing 2018 - San Francisco, United States
Duration: 2018 Jan 282018 Feb 1

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferencePhotons Plus Ultrasound: Imaging and Sensing 2018
Country/TerritoryUnited States
CitySan Francisco


  • angiogenesis
  • handheld
  • multi-wavelength
  • optoacoustic imaging
  • three-dimensional

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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