TY - JOUR
T1 - Fluorometric Biosniffer Camera "sniff-Cam" for Direct Imaging of Gaseous Ethanol in Breath and Transdermal Vapor
AU - Arakawa, Takahiro
AU - Sato, Toshiyuki
AU - Iitani, Kenta
AU - Toma, Koji
AU - Mitsubayashi, Kohji
N1 - Funding Information:
This work is partly supported by Japan Society for the Promotion of Science (JSPS) KAKENHI Grant-in-Aid for Scientific Research (B) Grant Number 15H04013 by Ministry of Education, Culture, Sports, Science and Technology (MEXT).
Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/4/18
Y1 - 2017/4/18
N2 - Various volatile organic compounds can be found in human transpiration, breath and body odor. In this paper, a novel two-dimensional fluorometric imaging system, known as a "sniffer-cam" for ethanol vapor released from human breath and palm skin was constructed and validated. This imaging system measures ethanol vapor concentrations as intensities of fluorescence through an enzymatic reaction induced by alcohol dehydrogenase (ADH). The imaging system consisted of multiple ultraviolet light emitting diode (UV-LED) excitation sheet, an ADH enzyme immobilized mesh substrate and a high-sensitive CCD camera. This imaging system uses ADH for recognition of ethanol vapor. It measures ethanol vapor by measuring fluorescence of nicotinamide adenine dinucleotide (NADH), which is produced by an enzymatic reaction on the mesh. This NADH fluorometric imaging system achieved the two-dimensional real-time imaging of ethanol vapor distribution (0.5-200 ppm). The system showed rapid and accurate responses and a visible measurement, which could lead to an analysis of metabolism function at real time in the near future.
AB - Various volatile organic compounds can be found in human transpiration, breath and body odor. In this paper, a novel two-dimensional fluorometric imaging system, known as a "sniffer-cam" for ethanol vapor released from human breath and palm skin was constructed and validated. This imaging system measures ethanol vapor concentrations as intensities of fluorescence through an enzymatic reaction induced by alcohol dehydrogenase (ADH). The imaging system consisted of multiple ultraviolet light emitting diode (UV-LED) excitation sheet, an ADH enzyme immobilized mesh substrate and a high-sensitive CCD camera. This imaging system uses ADH for recognition of ethanol vapor. It measures ethanol vapor by measuring fluorescence of nicotinamide adenine dinucleotide (NADH), which is produced by an enzymatic reaction on the mesh. This NADH fluorometric imaging system achieved the two-dimensional real-time imaging of ethanol vapor distribution (0.5-200 ppm). The system showed rapid and accurate responses and a visible measurement, which could lead to an analysis of metabolism function at real time in the near future.
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U2 - 10.1021/acs.analchem.6b04676
DO - 10.1021/acs.analchem.6b04676
M3 - Article
C2 - 28362084
AN - SCOPUS:85019984754
SN - 0003-2700
VL - 89
SP - 4495
EP - 4501
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 8
ER -