Functional optical coherence tomography of rat olfactory bulb with periodic odor stimulation

Hideyuki Watanabe, Uma Maheswari Rajagopalan, Yu Nakamichi, Kei M. Igarashi, Hirofumi Kadono, Manabu Tanifuji

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


In rodent olfactory bulb (OB), optical intrinsic signal imaging (OISI) is commonly used to investigate functional maps to odorant stimulations. However, in such studies, the spatial resolution in depth direction (z-axis) is lost because of the integration of light from different depths. To solve this problem, we propose functional optical coherence tomography (fOCT) with periodic stimulation and continuous recording. In fOCT experiments of in vivo rat OB, propionic acid and m-cresol were used as odor stimulus presentations. Such a periodic stimulation enabled us to detect the specific odorresponses from highly scattering brain tissue. Swept source OCT operating at a wavelength of 1334 nm and a frequency of 20 kHz, was employed with theoretical depth and lateral resolutions of 6.7 μm and 15.4 μm, respectively. We succeeded in visualizing 2D cross sectional fOCT map across the neural layer structure of OCT in vivo. The detected fOCT signals corresponded to a few glomeruli of the medial and lateral parts of dorsal OB. We also obtained 3D fOCT maps, which upon integration across z-axis agreed well with OISI results. We expect such an approach to open a window for investigating and possibly addressing toward inter/intra-layer connections at high resolutions in the future.

Original languageEnglish
Article number256150
Pages (from-to)841-854
Number of pages14
JournalBiomedical Optics Express
Issue number3
Publication statusPublished - 2016 Feb 10
Externally publishedYes


  • Frequency filtering
  • Functional monitoring and imaging
  • Image reconstruction techniques
  • Medical and biological imaging
  • Optical coherence tomography
  • Physiology
  • Threedimensional microscopy
  • Tissue characterization

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics


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