Genetic characterisation of genes involved in the upper pathway of carbazole metabolism from the putative Kordiimonas sp

Yoshihiko Ito; Rintaro Maeda; Kenichi Iwata; Toshio Omori

doi:10.1007/s10529-011-0639-z

Genetic characterisation of genes involved in the upper pathway of carbazole metabolism from the putative Kordiimonas sp

Yoshihiko Ito, Rintaro Maeda, Kenichi Iwata, Toshio Omori

Systems Engineering and Science

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The car genes from a carbazole (CAR)-degrading bacterium, Kordiimonas sp. OC9, were functionally and transcriptionally analysed. The enzymatic activity for the protein coded by carBaBb using pBOC93 (carAaAcBa), pBOC93-2 (carAaAcBb), and pBOC94 (carAaAcBaBb) was confirmed. Resting cells using Escherichia coli harbouring pBOC95 (carAaAcBaBbC) revealed the function of the carC gene product in the conversion of CAR to anthranilic acid by expressing it with CarAaAcBaBb. The pathway of CAR metabolism to anthranilic acid in marine CAR-degraders was elucidated. Transcriptional analysis using RT-PCR revealed that car genes are related to CAR degradation in response to CAR exposure in strain OC9. RT-PCR analysis of the operon structure showed that the car gene cluster of strain OC9 has two distinct operons in one car gene cluster. The localisation of the car gene cluster of strain OC9 was also determined.

Original language	English
Pages (from-to)	1859-1864
Number of pages	6
Journal	Biotechnology Letters
Volume	33
Issue number	9
DOIs	https://doi.org/10.1007/s10529-011-0639-z
Publication status	Published - 2011 Sept

Keywords

Bioremediation
Carbazole
Kordiimonas
Localisation of gene cluster
Transcriptional analysis

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/s10529-011-0639-z

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title = "Genetic characterisation of genes involved in the upper pathway of carbazole metabolism from the putative Kordiimonas sp",

abstract = "The car genes from a carbazole (CAR)-degrading bacterium, Kordiimonas sp. OC9, were functionally and transcriptionally analysed. The enzymatic activity for the protein coded by carBaBb using pBOC93 (carAaAcBa), pBOC93-2 (carAaAcBb), and pBOC94 (carAaAcBaBb) was confirmed. Resting cells using Escherichia coli harbouring pBOC95 (carAaAcBaBbC) revealed the function of the carC gene product in the conversion of CAR to anthranilic acid by expressing it with CarAaAcBaBb. The pathway of CAR metabolism to anthranilic acid in marine CAR-degraders was elucidated. Transcriptional analysis using RT-PCR revealed that car genes are related to CAR degradation in response to CAR exposure in strain OC9. RT-PCR analysis of the operon structure showed that the car gene cluster of strain OC9 has two distinct operons in one car gene cluster. The localisation of the car gene cluster of strain OC9 was also determined.",

keywords = "Bioremediation, Carbazole, Kordiimonas, Localisation of gene cluster, Transcriptional analysis",

author = "Yoshihiko Ito and Rintaro Maeda and Kenichi Iwata and Toshio Omori",

note = "Funding Information: Acknowledgments This research was supported by a Grant-in-Aid for Scientific Research (17380056 to T.O.) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.",

year = "2011",

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doi = "10.1007/s10529-011-0639-z",

language = "English",

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T1 - Genetic characterisation of genes involved in the upper pathway of carbazole metabolism from the putative Kordiimonas sp

AU - Ito, Yoshihiko

AU - Maeda, Rintaro

AU - Iwata, Kenichi

AU - Omori, Toshio

N1 - Funding Information: Acknowledgments This research was supported by a Grant-in-Aid for Scientific Research (17380056 to T.O.) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

PY - 2011/9

Y1 - 2011/9

N2 - The car genes from a carbazole (CAR)-degrading bacterium, Kordiimonas sp. OC9, were functionally and transcriptionally analysed. The enzymatic activity for the protein coded by carBaBb using pBOC93 (carAaAcBa), pBOC93-2 (carAaAcBb), and pBOC94 (carAaAcBaBb) was confirmed. Resting cells using Escherichia coli harbouring pBOC95 (carAaAcBaBbC) revealed the function of the carC gene product in the conversion of CAR to anthranilic acid by expressing it with CarAaAcBaBb. The pathway of CAR metabolism to anthranilic acid in marine CAR-degraders was elucidated. Transcriptional analysis using RT-PCR revealed that car genes are related to CAR degradation in response to CAR exposure in strain OC9. RT-PCR analysis of the operon structure showed that the car gene cluster of strain OC9 has two distinct operons in one car gene cluster. The localisation of the car gene cluster of strain OC9 was also determined.

AB - The car genes from a carbazole (CAR)-degrading bacterium, Kordiimonas sp. OC9, were functionally and transcriptionally analysed. The enzymatic activity for the protein coded by carBaBb using pBOC93 (carAaAcBa), pBOC93-2 (carAaAcBb), and pBOC94 (carAaAcBaBb) was confirmed. Resting cells using Escherichia coli harbouring pBOC95 (carAaAcBaBbC) revealed the function of the carC gene product in the conversion of CAR to anthranilic acid by expressing it with CarAaAcBaBb. The pathway of CAR metabolism to anthranilic acid in marine CAR-degraders was elucidated. Transcriptional analysis using RT-PCR revealed that car genes are related to CAR degradation in response to CAR exposure in strain OC9. RT-PCR analysis of the operon structure showed that the car gene cluster of strain OC9 has two distinct operons in one car gene cluster. The localisation of the car gene cluster of strain OC9 was also determined.

KW - Bioremediation

KW - Carbazole

KW - Kordiimonas

KW - Localisation of gene cluster

KW - Transcriptional analysis

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Genetic characterisation of genes involved in the upper pathway of carbazole metabolism from the putative Kordiimonas sp

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Keywords

ASJC Scopus subject areas

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