Global analysis of shear stress-responsive genes in vascular endothelial cells.

Norihiko Ohura, Kimiko Yamamoto, Sigeru Ichioka, Takaaki Sokabe, Hideki Nakatsuka, Atsushi Baba, Masahiro Shibata, Takashi Nakatsuka, Kiyonori Harii, Youichiro Wada, Takahide Kohro, Tatsuhiko Kodama, Joji Ando

Research output: Contribution to journalArticlepeer-review

129 Citations (Scopus)


DNA microarray gene expression analysis was conducted in human umbilical vein endothelial cells (HUVECs) and coronary artery endothelial cells (HCAECs) exposed to laminar or turbulent shear stress. Approximately 3% of the total 5600 gene in HUVECs and HCAECs increased their expression more than two-fold or decreased it to less than half the static control in response to an arterial level of laminar shear stress (15 dynes/cm(2) for 24 hours). The proportions of shear-stress-responsive genes decreased to around 2% under the venous level of laminar shear stress (1.5 dynes/cm(2)) in both cell lines. Turbulent shear stress of 1.5 dynes/cm(2) altered the expression of 1.1% of all genes in the HCAECs. Laminar shear stress, but not turbulent shear stress, decreased the expression of a number of genes involved in DNA synthesis and the cell cycle in both HUVECs and HCAECs. Clustering analysis showed a variety of temporal profiles of gene expression in HUVECs exposed to laminar shear stress of 15 dynes/cm(2) for 3, 6, 12, 24, and 48 hours. Turbulent shear stress affected expression of many genes that play a role in vascular remodeling, including genes encoding plasminogen activators and their inhibitor, endothelin-1, transforming growth factor-beta, collagen type IV, and ephrin A1.

Original languageEnglish
Pages (from-to)304-313
Number of pages10
JournalJournal of atherosclerosis and thrombosis
Issue number5
Publication statusPublished - 2003
Externally publishedYes

ASJC Scopus subject areas

  • Internal Medicine
  • Cardiology and Cardiovascular Medicine
  • Biochemistry, medical


Dive into the research topics of 'Global analysis of shear stress-responsive genes in vascular endothelial cells.'. Together they form a unique fingerprint.

Cite this