In situ control of cell adhesion using photoresponsive culture surface

Jun Ichi Edahiro, Kimio Sumaru, Yuichi Tada, Katsuhide Ohi, Toshiyuki Takagi, Mitsuyoshi Kameda, Toshio Shinbo, Toshiyuki Kanamori, Yasuo Yoshimi

Research output: Contribution to journalArticlepeer-review

184 Citations (Scopus)


A photoresponsive culture surface (PRCS) allowing photocontrol of cell adhesion was prepared with a novel polymer material composed of poly(N-isopropylacrylamide) having spiropyran chromophores as side chains. Cell adhesion of the surface was drastically enhanced by the irradiation with ultraviolet (UV) light (wavelength: 365 nm); after subsequent cooling and washing on ice, many cells remained in the irradiated region, whereas most cells were removed from the nonirradiated region. The cell adhesion of the PRCS, which had been enhanced by previous UV irradiation, was reset by the visible light irradiation (wavelength 400-440 nm) and the annealing at 37 °C for 2 h. Also it was confirmed that the regional control of cell adhesion was induced several times by repeating the same series of operations. Further, living cell patterning with the 200 μm line width was produced readily by projecting UV light along a micropattern on the PRCS on which the living cells had been seeded uniformly in advance. By using a fluorescent probe that stains living cells only, it was confirmed that the cells maintained sufficient viability even after UV light irradiation followed by cooling and washing.

Original languageEnglish
Pages (from-to)970-974
Number of pages5
Issue number2
Publication statusPublished - 2005 Mar 1

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry


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