TY - JOUR
T1 - In vitro cytotoxicity of carbon black nanoparticles synthesized from solution plasma on human lung fibroblast cells
AU - Panomsuwan, Gasidit
AU - Chokradjaroen, Chayanaphat
AU - Rujiravanit, Ratana
AU - Ueno, Tomonaga
AU - Saito, Nagahiro
N1 - Funding Information:
The authors wish to thank Dr. Sewan Theeramunkong of the Faculty of Pharmacy, Thammasat University for her technical assistance and the Drug Discovery and Development Center at Thammasat University for providing all the facilities that were used for the cytotoxicity tests. This work was partially supported by JST=CREST (Grant No. GJPMJCR12L1).
Publisher Copyright:
© 2018 The Japan Society of Applied Physics.
PY - 2018/1
Y1 - 2018/1
N2 - Carbon black nanoparticles (CB-NPs) have been synthesized from liquid benzene by a solution plasma method at room temperature and atmospheric pressure. The morphological observation by scanning electron microscopy revealed the agglomeration of aggregated fine particles. The synthesized CB-NPs were predominantly amorphous as confirmed by X-ray diffraction. The in vitro cytotoxicity of CB-NPs on the human lung fibroblast (MRC-5) cell line was assessed by the 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and systematically compared with those of two types of commercial carbon blacks (i.e., Vulcan XC-72 and Ketjenblack EC-600JD). Cell viabilities were studied at different concentrations of 32.5, 65, 125, and 250 μg/mL. It was found that the CB-NPs derived from solution plasma exhibited a lower cytotoxicity on the MRC-5 cells than the other two comparative carbon blacks. The viability of MRC-5 cells exposed to CB-NPs remained higher than 90% even at a high concentration of 250 μg/mL. This result preliminarily confirmed the biosafety and potential use of CB-NPs in the field of biological applications.
AB - Carbon black nanoparticles (CB-NPs) have been synthesized from liquid benzene by a solution plasma method at room temperature and atmospheric pressure. The morphological observation by scanning electron microscopy revealed the agglomeration of aggregated fine particles. The synthesized CB-NPs were predominantly amorphous as confirmed by X-ray diffraction. The in vitro cytotoxicity of CB-NPs on the human lung fibroblast (MRC-5) cell line was assessed by the 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and systematically compared with those of two types of commercial carbon blacks (i.e., Vulcan XC-72 and Ketjenblack EC-600JD). Cell viabilities were studied at different concentrations of 32.5, 65, 125, and 250 μg/mL. It was found that the CB-NPs derived from solution plasma exhibited a lower cytotoxicity on the MRC-5 cells than the other two comparative carbon blacks. The viability of MRC-5 cells exposed to CB-NPs remained higher than 90% even at a high concentration of 250 μg/mL. This result preliminarily confirmed the biosafety and potential use of CB-NPs in the field of biological applications.
UR - http://www.scopus.com/inward/record.url?scp=85040322317&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85040322317&partnerID=8YFLogxK
U2 - 10.7567/JJAP.57.0102BG
DO - 10.7567/JJAP.57.0102BG
M3 - Review article
AN - SCOPUS:85040322317
SN - 0021-4922
VL - 57
JO - Japanese Journal of Applied Physics
JF - Japanese Journal of Applied Physics
IS - 1
M1 - 0102BG
ER -