TY - GEN
T1 - In vivo microvascular oxygen measurements by phosphorescence quenching technique
AU - Shibata, M.
AU - Ichioka, S.
PY - 2006
Y1 - 2006
N2 - To clarify the oxygen transport across the microvessels and oxygen distribution in the skeletal muscle, we have newly designed an intravital laser microscopy with oxygen-dependent quenching of phosphorescence technique to determine both the microvascular and the tissue pO2. After injecting the phosphorescent probe into systemic blood, phosphorescence excited by a N2/dye pulse laser was detected with a photomultiplier over a 10 μm in diameter area. In vivo oxygen tension (pO2) measurements revealed the efficiency of this method. This method was then used to measure the pO2 of microvessels with different diameters (40-130 μm) and of interstitial spaces in rat cremaster muscle. These measurements showed a significant reduction of pO2 levels in arterioles of different orders and the presence of large pO2 gradients at the blood/tissue interface of arterioles. These findings suggest that oxygen can be released from larger arterioles and O2 consumption of rat cremaster muscle might be high.
AB - To clarify the oxygen transport across the microvessels and oxygen distribution in the skeletal muscle, we have newly designed an intravital laser microscopy with oxygen-dependent quenching of phosphorescence technique to determine both the microvascular and the tissue pO2. After injecting the phosphorescent probe into systemic blood, phosphorescence excited by a N2/dye pulse laser was detected with a photomultiplier over a 10 μm in diameter area. In vivo oxygen tension (pO2) measurements revealed the efficiency of this method. This method was then used to measure the pO2 of microvessels with different diameters (40-130 μm) and of interstitial spaces in rat cremaster muscle. These measurements showed a significant reduction of pO2 levels in arterioles of different orders and the presence of large pO2 gradients at the blood/tissue interface of arterioles. These findings suggest that oxygen can be released from larger arterioles and O2 consumption of rat cremaster muscle might be high.
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U2 - 10.1109/MHS.2006.320286
DO - 10.1109/MHS.2006.320286
M3 - Conference contribution
AN - SCOPUS:50449087031
SN - 1424407176
SN - 9781424407170
T3 - 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
BT - 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
T2 - 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
Y2 - 5 November 2006 through 8 November 2006
ER -