Localization of Gamma-Glutamyl Transpeptidase in the Proliferative State of Liver An Enzyme Histochemical and Immunohistochemical Study

The appearance and distribution of 7-glutamyl transpetidase (7-GTP) in the proliferative state of the liver were investigated by the methods of enzyme histochemistry and immunohistochemistry with monoclonal antibodies which specifically react with a heavy subunit of 7-GTP. In the fatal and neonatal liver, a positive reaction was observed along the luminal border of the interlobular bile ducts and that of the bile canaliculi in zones 1 and 2 of Rappaport. On the other hand, after partial hepatectomy, 7-GTP was localized along the luminal border of the interlobular bile ducts and that of the bile canaliculi in zone 1 of Rappaport. Both the enzyme histochemical and immunohistochemical methods yielded almost the same results for the distribution of 7-GTP.