Micro ELISA chip to Detect Multiple Allergic Proteins Simultaneously

Yuki Abe; Tadahiro Hasegawa; Mitsuo Yamasita

doi:10.1109/SII.2019.8700438

Micro ELISA chip to Detect Multiple Allergic Proteins Simultaneously

Yuki Abe, Tadahiro Hasegawa, Mitsuo Yamasita

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

The ELISA (Enzyme-Linked Immuno Sorbent Assay) chip to detect multiple allergic proteins simultaneously was developed successfully. This chip consists of multiple nitrocellulose membrane with 1mm diameter that immobilized several kinds of allergic proteins with 50ng amount only is fabricated using the multi-layer lithography. The experimental results demonstrated that 1) the specific allergen protein, and 2) the quantitative change of the antigen-antibody reaction depending on amount of antibody were able to be detected without contamination. The detection procedure that is only to inject antibodies in order into the chip is simple. The time for the whole detection procedure is short with 16 min. This ELISA chip that can specify the allergen protein that causes the patient's allergic disease is useful for the hyposensitization therapy.

Original language	English
Title of host publication	Proceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	675-678
Number of pages	4
ISBN (Electronic)	9781538636152
DOIs	https://doi.org/10.1109/SII.2019.8700438
Publication status	Published - 2019 Apr 25
Event	2019 IEEE/SICE International Symposium on System Integration, SII 2019 - Paris, France Duration: 2019 Jan 14 → 2019 Jan 16

Publication series

Name	Proceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019

Conference

Conference	2019 IEEE/SICE International Symposium on System Integration, SII 2019
Country/Territory	France
City	Paris
Period	19/1/14 → 19/1/16

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Electrical and Electronic Engineering

Access to Document

10.1109/SII.2019.8700438

Cite this

Abe, Y., Hasegawa, T., & Yamasita, M. (2019). Micro ELISA chip to Detect Multiple Allergic Proteins Simultaneously. In Proceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019 (pp. 675-678). [8700438] (Proceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/SII.2019.8700438

Micro ELISA chip to Detect Multiple Allergic Proteins Simultaneously. / Abe, Yuki; Hasegawa, Tadahiro; Yamasita, Mitsuo.
Proceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019. Institute of Electrical and Electronics Engineers Inc., 2019. p. 675-678 8700438 (Proceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abe, Y, Hasegawa, T & Yamasita, M 2019, Micro ELISA chip to Detect Multiple Allergic Proteins Simultaneously. in Proceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019., 8700438, Proceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019, Institute of Electrical and Electronics Engineers Inc., pp. 675-678, 2019 IEEE/SICE International Symposium on System Integration, SII 2019, Paris, France, 19/1/14. https://doi.org/10.1109/SII.2019.8700438

Abe Y, Hasegawa T, Yamasita M. Micro ELISA chip to Detect Multiple Allergic Proteins Simultaneously. In Proceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019. Institute of Electrical and Electronics Engineers Inc. 2019. p. 675-678. 8700438. (Proceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019). doi: 10.1109/SII.2019.8700438

Abe, Yuki ; Hasegawa, Tadahiro ; Yamasita, Mitsuo. / Micro ELISA chip to Detect Multiple Allergic Proteins Simultaneously. Proceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019. Institute of Electrical and Electronics Engineers Inc., 2019. pp. 675-678 (Proceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019).

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title = "Micro ELISA chip to Detect Multiple Allergic Proteins Simultaneously",

abstract = "The ELISA (Enzyme-Linked Immuno Sorbent Assay) chip to detect multiple allergic proteins simultaneously was developed successfully. This chip consists of multiple nitrocellulose membrane with 1mm diameter that immobilized several kinds of allergic proteins with 50ng amount only is fabricated using the multi-layer lithography. The experimental results demonstrated that 1) the specific allergen protein, and 2) the quantitative change of the antigen-antibody reaction depending on amount of antibody were able to be detected without contamination. The detection procedure that is only to inject antibodies in order into the chip is simple. The time for the whole detection procedure is short with 16 min. This ELISA chip that can specify the allergen protein that causes the patient's allergic disease is useful for the hyposensitization therapy.",

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Micro ELISA chip to Detect Multiple Allergic Proteins Simultaneously

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