Microfluidic cell culture system with on-chip hypoxic conditioning

Atsushi Takano; Masato Tanaka; Nobuyuki Futai

doi:10.1109/EMBC.2013.6610540

Microfluidic cell culture system with on-chip hypoxic conditioning

Atsushi Takano, Masato Tanaka, Nobuyuki Futai

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Citations (Scopus)

Abstract

We have demonstrated a portable microfluidic cell culture system with multi-gas (CO₂ and O₂) incubation which we can cultivate under hypoxia without bulky peripheral apparatus such as gas tanks, regulators, and flow controllers. The system contains a chip of 26 mm × 48 mm which is capable to diffuse CO₂ and absorb O₂ through a gas-permeable wall of nested media reservoir. The media was water-jacketed with aqueous solution containing 0.8 M sodium bicarbonate as CO₂ supply and 1M sodium ascorbate as oxygen scavenger. The partial CO₂ pressure (pCO₂) in media reservoir stabilized at least 10.2% ± 0.11% for at least 72 hours. The partial O₂ pressure (pO₂) in the media reservoir decreased to 4.2%. Portable on-chip hypoxic culture of SV40-T2 cells for 72 h was also demonstrated.

Original language	English
Title of host publication	2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013
Pages	4474-4477
Number of pages	4
DOIs	https://doi.org/10.1109/EMBC.2013.6610540
Publication status	Published - 2013
Event	2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013 - Osaka, Japan Duration: 2013 Jul 3 → 2013 Jul 7

Publication series

Name	Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ISSN (Print)	1557-170X

Conference

Conference	2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013
Country/Territory	Japan
City	Osaka
Period	13/7/3 → 13/7/7

ASJC Scopus subject areas

Signal Processing
Biomedical Engineering
Computer Vision and Pattern Recognition
Health Informatics

Access to Document

10.1109/EMBC.2013.6610540

Cite this

Takano, A., Tanaka, M., & Futai, N. (2013). Microfluidic cell culture system with on-chip hypoxic conditioning. In 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013 (pp. 4474-4477). [6610540] (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). https://doi.org/10.1109/EMBC.2013.6610540

Microfluidic cell culture system with on-chip hypoxic conditioning. / Takano, Atsushi; Tanaka, Masato; Futai, Nobuyuki.
2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013. 2013. p. 4474-4477 6610540 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Takano, A, Tanaka, M & Futai, N 2013, Microfluidic cell culture system with on-chip hypoxic conditioning. in 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013., 6610540, Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS, pp. 4474-4477, 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013, Osaka, Japan, 13/7/3. https://doi.org/10.1109/EMBC.2013.6610540

Takano A, Tanaka M, Futai N. Microfluidic cell culture system with on-chip hypoxic conditioning. In 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013. 2013. p. 4474-4477. 6610540. (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). doi: 10.1109/EMBC.2013.6610540

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abstract = "We have demonstrated a portable microfluidic cell culture system with multi-gas (CO2 and O2) incubation which we can cultivate under hypoxia without bulky peripheral apparatus such as gas tanks, regulators, and flow controllers. The system contains a chip of 26 mm × 48 mm which is capable to diffuse CO2 and absorb O2 through a gas-permeable wall of nested media reservoir. The media was water-jacketed with aqueous solution containing 0.8 M sodium bicarbonate as CO2 supply and 1M sodium ascorbate as oxygen scavenger. The partial CO2 pressure (pCO2) in media reservoir stabilized at least 10.2% ± 0.11% for at least 72 hours. The partial O2 pressure (pO2) in the media reservoir decreased to 4.2%. Portable on-chip hypoxic culture of SV40-T2 cells for 72 h was also demonstrated.",

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Microfluidic cell culture system with on-chip hypoxic conditioning

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