@inproceedings{2c51e1dad9d545d1afd95689fc64005a,
title = "Microfluidic culture of primary neurons with on-chip hypoxic conditioning",
abstract = "We have developed a pocket-sized microfluidic cell culture chip that enables on-chip multi-gas (CO2 and O2) control without any feedback control or external gas supply. Elevated CO2 levels (∼7%) and hypoxic conditions (O2 levels, ∼5%) were simultaneously achieved on the chip by maintaining the bottom of the chip at 37 °C and filling an adjacent reservoir with a bicarbonate/carbonate/ascorbate solution. We have also successfully cultured primary chick embryo neurons onchip under hypoxic conditions for 3 days. The neurons cultured under hypoxia showed inhibited neurite outgrowth.",
keywords = "Hypoxic conditioning, Long-term culture, On-chip multi-gas incubation, Primary neuron",
author = "Atsushi Takano and Saori Inomata and Masato Tanaka and Nobuyuki Futai",
year = "2013",
language = "English",
isbn = "9781632666246",
series = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
publisher = "Chemical and Biological Microsystems Society",
pages = "419--421",
booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
note = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 ; Conference date: 27-10-2013 Through 31-10-2013",
}