Molecular cloning and expression of Xenopus laevis Requiem cDNA

Masahiro Konishi; Yoshiki Hiraoka; Motoyuki Ogawa; Yukinao Sakai; Hiromasa Ishii; Sadakazu Aiso

doi:10.1016/S0167-4781(99)00031-7

Molecular cloning and expression of Xenopus laevis Requiem cDNA

Masahiro Konishi, Yoshiki Hiraoka, Motoyuki Ogawa, Yukinao Sakai, Hiromasa Ishii, Sadakazu Aiso

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Requiem is an apoptosis-associated gene, which was originally identified in mouse (T.G. Gabig et al., J. Biol. Chem. 269 (1994) 29515-29519). In this study, we isolated five independent cDNA clones for frog Requiem (xReq) from Xenopus laevis ovary. Sequence analysis of the multiple cDNAs has suggested that Xenopus genome contains at least two non-allelic copies of the xReq gene. The amino acid sequence deduced from the cDNAs contained a single Kruppel-type zinc-finger motif and two PHD-finger motifs. Northern analysis revealed that the ovary expressed xReq transcripts with different sizes.

Original language	English
Pages (from-to)	172-176
Number of pages	5
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1445
Issue number	1
DOIs	https://doi.org/10.1016/S0167-4781(99)00031-7
Publication status	Published - 1999 Apr 14
Externally published	Yes

Keywords

Apoptosis
Embryo
Kruppel
Requiem
Zinc-finger

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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title = "Molecular cloning and expression of Xenopus laevis Requiem cDNA",

abstract = "Requiem is an apoptosis-associated gene, which was originally identified in mouse (T.G. Gabig et al., J. Biol. Chem. 269 (1994) 29515-29519). In this study, we isolated five independent cDNA clones for frog Requiem (xReq) from Xenopus laevis ovary. Sequence analysis of the multiple cDNAs has suggested that Xenopus genome contains at least two non-allelic copies of the xReq gene. The amino acid sequence deduced from the cDNAs contained a single Kruppel-type zinc-finger motif and two PHD-finger motifs. Northern analysis revealed that the ovary expressed xReq transcripts with different sizes.",

keywords = "Apoptosis, Embryo, Kruppel, Requiem, Zinc-finger",

author = "Masahiro Konishi and Yoshiki Hiraoka and Motoyuki Ogawa and Yukinao Sakai and Hiromasa Ishii and Sadakazu Aiso",

note = "Funding Information: This work was supported in part by a National Grant-in-Aid for the Establishment of High-Tech Research Center in a Private University from Ministry of Education, Science and Culture to S.A., and Grants-in-Aid from Ministry of Education, Science and Culture to S.A. and Y.H., by Keio Gakuji Fukuzawa Memorial Funds for Advancement of Education and Research and Keio Gakuji Academic Developmental Funds from Keio University to S.A. and Y.H.",

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doi = "10.1016/S0167-4781(99)00031-7",

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T1 - Molecular cloning and expression of Xenopus laevis Requiem cDNA

AU - Konishi, Masahiro

AU - Hiraoka, Yoshiki

AU - Ogawa, Motoyuki

AU - Sakai, Yukinao

AU - Ishii, Hiromasa

AU - Aiso, Sadakazu

N1 - Funding Information: This work was supported in part by a National Grant-in-Aid for the Establishment of High-Tech Research Center in a Private University from Ministry of Education, Science and Culture to S.A., and Grants-in-Aid from Ministry of Education, Science and Culture to S.A. and Y.H., by Keio Gakuji Fukuzawa Memorial Funds for Advancement of Education and Research and Keio Gakuji Academic Developmental Funds from Keio University to S.A. and Y.H.

PY - 1999/4/14

Y1 - 1999/4/14

N2 - Requiem is an apoptosis-associated gene, which was originally identified in mouse (T.G. Gabig et al., J. Biol. Chem. 269 (1994) 29515-29519). In this study, we isolated five independent cDNA clones for frog Requiem (xReq) from Xenopus laevis ovary. Sequence analysis of the multiple cDNAs has suggested that Xenopus genome contains at least two non-allelic copies of the xReq gene. The amino acid sequence deduced from the cDNAs contained a single Kruppel-type zinc-finger motif and two PHD-finger motifs. Northern analysis revealed that the ovary expressed xReq transcripts with different sizes.

AB - Requiem is an apoptosis-associated gene, which was originally identified in mouse (T.G. Gabig et al., J. Biol. Chem. 269 (1994) 29515-29519). In this study, we isolated five independent cDNA clones for frog Requiem (xReq) from Xenopus laevis ovary. Sequence analysis of the multiple cDNAs has suggested that Xenopus genome contains at least two non-allelic copies of the xReq gene. The amino acid sequence deduced from the cDNAs contained a single Kruppel-type zinc-finger motif and two PHD-finger motifs. Northern analysis revealed that the ovary expressed xReq transcripts with different sizes.

KW - Apoptosis

KW - Embryo

KW - Kruppel

KW - Requiem

KW - Zinc-finger

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JO - Biochimica et Biophysica Acta - Gene Structure and Expression

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Molecular cloning and expression of Xenopus laevis Requiem cDNA

Abstract

Keywords

ASJC Scopus subject areas

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