TY - JOUR
T1 - Molecular cloning of a new class of cartilage-specific matrix, chondromodulin-I, which stimulates growth of cultured chondrocytes
AU - Hiraki, Yuji
AU - Tanaka, Hideho
AU - Inoue, Hiroyuki
AU - Kondo, Jun
AU - Kamizono, Akihito
AU - Suzuki, Fujio
N1 - Funding Information:
We thank Y. Sanma, S. Megumi and A. Asada (Osaka University, Faculty of Dentistry) for technical assistance; Y. Teranishi (Mitsubishi Kasei Corp.) for encouragement; Y. Miyazaki (Mitsubishi Kasei Corp., Nakashibetsu) for supplying bovine fetal cartilage and E. Ichihara for reviewing the manuscript. This work was partly supported by Kowa Life Science Foundation and @an&n-Aid from the Ministry of Education, Science and Culture of Japan.
PY - 1991/3/29
Y1 - 1991/3/29
N2 - Here we report the structure and bioactivity of 25 kDa glycoprotein (chondromodulin-I) as a tissue-specific functional matrix component identified and cloned for the first time. Chondromodulin-I purified from fetal bovine cartilage markedly stimulated DNA synthesis of cultured growth-plate chondrocytes in the presence of basic fibroblast growth factor (FGF). Bovine chondromodulin-I cDNA revealed that the mature protein consists of 121 amino acids with three possible glycosylation sites and is coded as the C-terminal part of a larger precursor. On northern blot analysis, expression of chondromodulin-I mRNA was observed only in cartilage.
AB - Here we report the structure and bioactivity of 25 kDa glycoprotein (chondromodulin-I) as a tissue-specific functional matrix component identified and cloned for the first time. Chondromodulin-I purified from fetal bovine cartilage markedly stimulated DNA synthesis of cultured growth-plate chondrocytes in the presence of basic fibroblast growth factor (FGF). Bovine chondromodulin-I cDNA revealed that the mature protein consists of 121 amino acids with three possible glycosylation sites and is coded as the C-terminal part of a larger precursor. On northern blot analysis, expression of chondromodulin-I mRNA was observed only in cartilage.
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U2 - 10.1016/0006-291X(91)91660-5
DO - 10.1016/0006-291X(91)91660-5
M3 - Article
C2 - 1709014
AN - SCOPUS:0025881511
SN - 0006-291X
VL - 175
SP - 971
EP - 977
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -