On-chip multi-gas incubation for microfluidic cell cultures under hypoxia

Atsushi Takano, Masato Tanaka, Nobuyuki Futai

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


We developed a simple system that regulates CO2 and O2 levels within a microfluidic chip. This system enables long-term cell culture under hypoxic conditions without the need of a CO2 incubator or a multi-gas incubator. Hypoxic conditions were generated using a miniature water jacket containing dissolved ascorbate as an oxygen scavenger. Formulations of the water jacket were determined that enables both 5% pCO2 and desired pO2 levels ranging from 5 to 15%. We also cultured PC-12 cells and primary neuronal cells from chick embryos under hypoxia and observed hypoxia-induced cell death and inhibition of neurite outgrowth.

Original languageEnglish
Article number061101
Issue number6
Publication statusPublished - 2014 Nov 25

ASJC Scopus subject areas

  • Biomedical Engineering
  • General Materials Science
  • Condensed Matter Physics
  • Fluid Flow and Transfer Processes
  • Colloid and Surface Chemistry


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