Overexpression of CR/periphilin downregulates Cdc7 expression and induces S-phase arrest

Megumi Kurita; Hiroaki Suzuki; Hisao Masai; Kiyohisa Mizumoto; Etsuro Ogata; Ikuo Nishimoto; Sadakazu Aiso; Masaaki Matsuoka

doi:10.1016/j.bbrc.2004.09.083

Overexpression of CR/periphilin downregulates Cdc7 expression and induces S-phase arrest

Megumi Kurita, Hiroaki Suzuki, Hisao Masai, Kiyohisa Mizumoto, Etsuro Ogata, Ikuo Nishimoto, Sadakazu Aiso, Masaaki Matsuoka

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Cdc7 expression repressor (CR)/periphilin has been originally cloned as an interactor with periplakin, a precursor of the cornified cell envelope, and suggested to constitute a new type of nuclear matrix. We here show that CR/periphilin is a ubiquitously expressed nuclear protein with speckled distribution. Overexpression of CR/periphilin induces S-phase arrest. Analysis of expression of regulators involved in DNA replication has revealed that both mRNA and protein expression of Cdc7, a regulator of the initiation and continuation of DNA replication, are markedly downregulated by overexpression of CR/periphilin. However, co-expression of Cdc7 only marginally rescues S-phase arrest induced by CR, indicating that CR retards S-phase progression by modifying expression of some genes including Cdc7, which are involved in progression of DNA replication or coordination of DNA replication and S-phase progression.

Original language	English
Pages (from-to)	554-561
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	324
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2004.09.083
Publication status	Published - 2004 Nov 12
Externally published	Yes

Keywords

CR
Cdc7
Cell cycle
Periphilin
S-phase arrest

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2004.09.083

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title = "Overexpression of CR/periphilin downregulates Cdc7 expression and induces S-phase arrest",

abstract = "Cdc7 expression repressor (CR)/periphilin has been originally cloned as an interactor with periplakin, a precursor of the cornified cell envelope, and suggested to constitute a new type of nuclear matrix. We here show that CR/periphilin is a ubiquitously expressed nuclear protein with speckled distribution. Overexpression of CR/periphilin induces S-phase arrest. Analysis of expression of regulators involved in DNA replication has revealed that both mRNA and protein expression of Cdc7, a regulator of the initiation and continuation of DNA replication, are markedly downregulated by overexpression of CR/periphilin. However, co-expression of Cdc7 only marginally rescues S-phase arrest induced by CR, indicating that CR retards S-phase progression by modifying expression of some genes including Cdc7, which are involved in progression of DNA replication or coordination of DNA replication and S-phase progression.",

keywords = "CR, Cdc7, Cell cycle, Periphilin, S-phase arrest",

author = "Megumi Kurita and Hiroaki Suzuki and Hisao Masai and Kiyohisa Mizumoto and Etsuro Ogata and Ikuo Nishimoto and Sadakazu Aiso and Masaaki Matsuoka",

note = "Funding Information: We thank Dr. Masaki Kitajima for essential help. We are indebted to Dr. Kenzo Terashita. We are indebted to Tomo Yoshida, Kazumi Nishihara, Takako Hiraki, and Dovie Wylie for expert technical assistance. This work was supported in part by the Naito Foundation and the Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.",

year = "2004",

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doi = "10.1016/j.bbrc.2004.09.083",

language = "English",

volume = "324",

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journal = "Biochemical and Biophysical Research Communications",

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T1 - Overexpression of CR/periphilin downregulates Cdc7 expression and induces S-phase arrest

AU - Kurita, Megumi

AU - Suzuki, Hiroaki

AU - Masai, Hisao

AU - Mizumoto, Kiyohisa

AU - Ogata, Etsuro

AU - Nishimoto, Ikuo

AU - Aiso, Sadakazu

AU - Matsuoka, Masaaki

N1 - Funding Information: We thank Dr. Masaki Kitajima for essential help. We are indebted to Dr. Kenzo Terashita. We are indebted to Tomo Yoshida, Kazumi Nishihara, Takako Hiraki, and Dovie Wylie for expert technical assistance. This work was supported in part by the Naito Foundation and the Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.

PY - 2004/11/12

Y1 - 2004/11/12

N2 - Cdc7 expression repressor (CR)/periphilin has been originally cloned as an interactor with periplakin, a precursor of the cornified cell envelope, and suggested to constitute a new type of nuclear matrix. We here show that CR/periphilin is a ubiquitously expressed nuclear protein with speckled distribution. Overexpression of CR/periphilin induces S-phase arrest. Analysis of expression of regulators involved in DNA replication has revealed that both mRNA and protein expression of Cdc7, a regulator of the initiation and continuation of DNA replication, are markedly downregulated by overexpression of CR/periphilin. However, co-expression of Cdc7 only marginally rescues S-phase arrest induced by CR, indicating that CR retards S-phase progression by modifying expression of some genes including Cdc7, which are involved in progression of DNA replication or coordination of DNA replication and S-phase progression.

AB - Cdc7 expression repressor (CR)/periphilin has been originally cloned as an interactor with periplakin, a precursor of the cornified cell envelope, and suggested to constitute a new type of nuclear matrix. We here show that CR/periphilin is a ubiquitously expressed nuclear protein with speckled distribution. Overexpression of CR/periphilin induces S-phase arrest. Analysis of expression of regulators involved in DNA replication has revealed that both mRNA and protein expression of Cdc7, a regulator of the initiation and continuation of DNA replication, are markedly downregulated by overexpression of CR/periphilin. However, co-expression of Cdc7 only marginally rescues S-phase arrest induced by CR, indicating that CR retards S-phase progression by modifying expression of some genes including Cdc7, which are involved in progression of DNA replication or coordination of DNA replication and S-phase progression.

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KW - Cell cycle

KW - Periphilin

KW - S-phase arrest

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Overexpression of CR/periphilin downregulates Cdc7 expression and induces S-phase arrest

Abstract

Keywords

ASJC Scopus subject areas

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