Photodynamic assistance increases the efficiency of the process of microinjection in animal cells

Takashi K. Saito; Hitoshi Muguruma; Kunihiko Mabuchi

doi:10.1023/A:1014072904009

Photodynamic assistance increases the efficiency of the process of microinjection in animal cells

Takashi K. Saito, Hitoshi Muguruma, Kunihiko Mabuchi

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

A microinjection process is described that involves the photodynamically-assisted puncture of an animal cell membrane by means of a brief and partial degeneration resulting from cell membrane damage. The ratio of dye-injected rat pheochromocytoma cells that became detached three and six days after photodynamically-assisted microinjection was 9%; however, it was 83% when a conventional mechanical microinjection process was used.

Original language	English
Pages (from-to)	309-314
Number of pages	6
Journal	Biotechnology Letters
Volume	24
Issue number	4
DOIs	https://doi.org/10.1023/A:1014072904009
Publication status	Published - 2002

Keywords

Detachment assay
Microinjection
Photodynamic membrane damage
Rat PC12 cell line
α-terthienyl derivative

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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title = "Photodynamic assistance increases the efficiency of the process of microinjection in animal cells",

abstract = "A microinjection process is described that involves the photodynamically-assisted puncture of an animal cell membrane by means of a brief and partial degeneration resulting from cell membrane damage. The ratio of dye-injected rat pheochromocytoma cells that became detached three and six days after photodynamically-assisted microinjection was 9%; however, it was 83% when a conventional mechanical microinjection process was used.",

keywords = "Detachment assay, Microinjection, Photodynamic membrane damage, Rat PC12 cell line, α-terthienyl derivative",

author = "Saito, {Takashi K.} and Hitoshi Muguruma and Kunihiko Mabuchi",

note = "Funding Information: This work was partly supported by a grant for {\textquoteleft}Research for the Future, Program No. 97I00401{\textquoteright} from the Japan Society for the Promotion of Science. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

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AU - Saito, Takashi K.

AU - Muguruma, Hitoshi

AU - Mabuchi, Kunihiko

N1 - Funding Information: This work was partly supported by a grant for ‘Research for the Future, Program No. 97I00401’ from the Japan Society for the Promotion of Science. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2002

Y1 - 2002

N2 - A microinjection process is described that involves the photodynamically-assisted puncture of an animal cell membrane by means of a brief and partial degeneration resulting from cell membrane damage. The ratio of dye-injected rat pheochromocytoma cells that became detached three and six days after photodynamically-assisted microinjection was 9%; however, it was 83% when a conventional mechanical microinjection process was used.

AB - A microinjection process is described that involves the photodynamically-assisted puncture of an animal cell membrane by means of a brief and partial degeneration resulting from cell membrane damage. The ratio of dye-injected rat pheochromocytoma cells that became detached three and six days after photodynamically-assisted microinjection was 9%; however, it was 83% when a conventional mechanical microinjection process was used.

KW - Detachment assay

KW - Microinjection

KW - Photodynamic membrane damage

KW - Rat PC12 cell line

KW - α-terthienyl derivative

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JO - Biotechnology Letters

JF - Biotechnology Letters

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ER -

Photodynamic assistance increases the efficiency of the process of microinjection in animal cells

Abstract

Keywords

ASJC Scopus subject areas

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