TY - JOUR
T1 - Precipitate-enhanced SAW immunosensor for sensitive monitoring of mite allergens
AU - Toma, Koji
AU - Oishi, Koki
AU - Kato, Misato
AU - Kurata, Kanako
AU - Yoshimura, Naoyuki
AU - Arakawa, Takahiro
AU - Yatsuda, Hiromi
AU - Kanamori, Kiyoko
AU - Mitsubayashi, Kohji
N1 - Funding Information:
This work was partly supported by Japan Society for the Promotion of Science (JSPS, Japan) KAKENHI Grant Number JP 16K18112 and 18K13763 , and the Ministry of Education, Culture, Sports, Science and Technology (MEXT, Japan) Special Funds for “Cooperative Research Project of Research Center for Biomedical Engineering”.
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/10/1
Y1 - 2019/10/1
N2 - Precipitates produced via horseradish peroxidase (HRP-) catalyzed reaction was exploited to enhance the sensitivity of a surface acoustic wave (SAW) immunosensor for monitoring airborne house dust mite (HDM) allergens. Three candidate substrates, 4-chloro-1-naphthol (4CN), 3,3′-diaminobenzidine (DAB) and 3,3′,5,5′-tetramethylbenzidine (TMB), were systematically compared in terms of signal amplification and suitability to rapid and repeated measurement. Each substrate solution was applied to the sensor surface where group 2 HDM allergens of Dermatophagoides farinae (Der f 2) were sandwiched by capture and HRP-conjugated detection antibodies. The signal amplification and sensor regeneration rate were evaluated from the sensor output induced by precipitated product of HRP-catalyzed reaction and the sensor signal recovery after the regeneration via various pH reagents, respectively. As a result, it was revealed that 4CN showed the highest surface regeneration rate among the three substrates with use of NaOH adjusted to pH 13 as well as the second largest enhancement of the sensor output. Use of 4CN enabled to enhance the sensor output by a factor of 13 for 1 ng/mL Der f 2 with respect to that without the HRP-catalyzed reaction. The limit of detection of the precipitate-enhanced SAW immunosensor was 35 pg/mL (equivalent to 2.3 pM), which was 1.4-fold and 206-fold better than those of ELISA and the SAW immunosensor without a precipitate, respectively. These indicate that exploiting 4CN under the optimal conditions in the SAW immunosensor realizes ultra-sensitive monitoring of HDM allergens.
AB - Precipitates produced via horseradish peroxidase (HRP-) catalyzed reaction was exploited to enhance the sensitivity of a surface acoustic wave (SAW) immunosensor for monitoring airborne house dust mite (HDM) allergens. Three candidate substrates, 4-chloro-1-naphthol (4CN), 3,3′-diaminobenzidine (DAB) and 3,3′,5,5′-tetramethylbenzidine (TMB), were systematically compared in terms of signal amplification and suitability to rapid and repeated measurement. Each substrate solution was applied to the sensor surface where group 2 HDM allergens of Dermatophagoides farinae (Der f 2) were sandwiched by capture and HRP-conjugated detection antibodies. The signal amplification and sensor regeneration rate were evaluated from the sensor output induced by precipitated product of HRP-catalyzed reaction and the sensor signal recovery after the regeneration via various pH reagents, respectively. As a result, it was revealed that 4CN showed the highest surface regeneration rate among the three substrates with use of NaOH adjusted to pH 13 as well as the second largest enhancement of the sensor output. Use of 4CN enabled to enhance the sensor output by a factor of 13 for 1 ng/mL Der f 2 with respect to that without the HRP-catalyzed reaction. The limit of detection of the precipitate-enhanced SAW immunosensor was 35 pg/mL (equivalent to 2.3 pM), which was 1.4-fold and 206-fold better than those of ELISA and the SAW immunosensor without a precipitate, respectively. These indicate that exploiting 4CN under the optimal conditions in the SAW immunosensor realizes ultra-sensitive monitoring of HDM allergens.
KW - Allergen
KW - House dust mite
KW - Immunosensor
KW - Precipitate
KW - Repeated immunoassay
KW - Surface acoustic wave
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U2 - 10.1016/j.snb.2019.05.056
DO - 10.1016/j.snb.2019.05.056
M3 - Article
AN - SCOPUS:85066931724
SN - 0925-4005
VL - 296
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
M1 - 126579
ER -