TY - JOUR
T1 - Repeated immunosensing by a dithiobis(succinimidyl propionate)-modified SAW device
AU - Toma, Koji
AU - Oishi, Koki
AU - Yoshimura, Naoyuki
AU - Arakawa, Takahiro
AU - Yatsuda, Hiromi
AU - Mitsubayashi, Kohji
N1 - Funding Information:
This work was partly supported by Japan Society for the Promotion of Science ( JSPS , Japan) KAKENHI Grant Number JP 16K18112 and 18K13763, and the Ministry of Education, Culture, Sports, Science and Technology ( MEXT , Japan) Special Funds for “Cooperative Research Project of Research Center for Biomedical Engineering”.
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/10/1
Y1 - 2019/10/1
N2 - The possibility of dithiobis(succinimidyl propionate) (DSP) for repeated immunoassay with a surface acoustic wave (SAW) immunosensor was explored. In the sensor, DSP was used to modify a gold-coated quartz sensing area of a SAW device by forming a self-assembled monolayer on the gold surface. In a model sandwich assay using mouse (mIgG) and anti-mouse (a-mIgG) antibodies, the primary antibody, mIgG, firstly reacted with N-hydroxysuccinimide ester groups of DSP and was immobilized on the SAW device to fabricate the SAW immunosensor. Optimization of adsorption time of mIgG revealed that both degrees of adsorption and immobilization of mIgG reached a saturation at 30 min although the immobilization was more dependent on the adsorption time. Through characterization of the DSP-modified SAW immunosensor, a high selectivity, with which no sensor output was observed from various kinds of secondary antibodies except for a-mIgG, along with 8-fold shorter measurement time (15 min) than that of enzyme-linked immunosorbent assay were obtained. Furthermore, 10 repeated measurement of a-mIgG demonstrated a high reproducibility of the sensor output (coefficient of variation of 7.0%). These validate the utility of DSP in the SAW immunosensor for rapid and repeated measurement of antigens.
AB - The possibility of dithiobis(succinimidyl propionate) (DSP) for repeated immunoassay with a surface acoustic wave (SAW) immunosensor was explored. In the sensor, DSP was used to modify a gold-coated quartz sensing area of a SAW device by forming a self-assembled monolayer on the gold surface. In a model sandwich assay using mouse (mIgG) and anti-mouse (a-mIgG) antibodies, the primary antibody, mIgG, firstly reacted with N-hydroxysuccinimide ester groups of DSP and was immobilized on the SAW device to fabricate the SAW immunosensor. Optimization of adsorption time of mIgG revealed that both degrees of adsorption and immobilization of mIgG reached a saturation at 30 min although the immobilization was more dependent on the adsorption time. Through characterization of the DSP-modified SAW immunosensor, a high selectivity, with which no sensor output was observed from various kinds of secondary antibodies except for a-mIgG, along with 8-fold shorter measurement time (15 min) than that of enzyme-linked immunosorbent assay were obtained. Furthermore, 10 repeated measurement of a-mIgG demonstrated a high reproducibility of the sensor output (coefficient of variation of 7.0%). These validate the utility of DSP in the SAW immunosensor for rapid and repeated measurement of antigens.
KW - Dithiobis(succinimidyl propionate)
KW - Immunosensor
KW - Repeated measurement
KW - Surface acoustic wave
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U2 - 10.1016/j.talanta.2019.05.080
DO - 10.1016/j.talanta.2019.05.080
M3 - Article
C2 - 31202338
AN - SCOPUS:85066154167
SN - 0039-9140
VL - 203
SP - 274
EP - 279
JO - Talanta
JF - Talanta
ER -