Sequential Injection Analysis of Butyrylcholinesterase Using Butyrylcholine Ion-Selective Electrode Detector

Narimasa Koizumi, Ryusei Nishiyama, Takashi Masadome

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

A new automated analytical method for the determination of Butyrylcholinesterase (BChE) based on sequential injection analysis (SIA) method using a butyrylcholine chloride (BuCh) ion-selective electrode (ISE) detector was developed. BuCh is degraded to choline and butyrate by the enzymatic activity of BChE, resulting in a decrease in BuCh concentration. By detecting this change in BuCh concentration with BuCh-ISE, BChE can be determined indirectly. In the present SIA method using the BuCh-ISE detector based on α-cyclodextrin membrane, BChE in the concentration range of 0.015–0.50 units/mL was determined at a BuCh concentration of 2.0 mmol dm−3 and a reaction time of 5.0 min. The detection limit for BChE is 4.8 × 10−3 units/mL.

Original languageEnglish
Article number097003
JournalElectrochemistry
Volume90
Issue number9
DOIs
Publication statusPublished - 2022

Keywords

  • Butyrylcholinesterase
  • Enzyme
  • Ion-Selective Electrode
  • Sequential Injection Analysis

ASJC Scopus subject areas

  • Electrochemistry

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