Simultaneous Analysis of Hydrochlorothiazide, Triamterene and Reserpine in Rat Plasma by HPLC and DSPE

Xiaogang Chen; Weidi Teng; Lei Miao; Yan Wu; Dazhi Chen; Liyan Huang; Jiahui Pan; Ning Wang; Jianzhang Fang; Yong Liang

doi:10.1007/s10337-016-3060-x

Simultaneous Analysis of Hydrochlorothiazide, Triamterene and Reserpine in Rat Plasma by HPLC and DSPE

Xiaogang Chen, Weidi Teng, Lei Miao, Yan Wu, Dazhi Chen, Liyan Huang, Jiahui Pan, Ning Wang, Jianzhang Fang, Yong Liang

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A dispersive solid-phase extraction (DSPE) method of mixing two different materials (C₁₈ and MAX) was developed as extraction procedure, which provided better extraction yields (>82.1 %) for all analytes and more appropriate sample purification from endogenous interference materials compared with common SPE. Analyte separation was achieved on a C₁₈ reversed phase column at the wavelength of 265 nm by high performance liquid chromatography (HPLC). The method was validated in terms of extraction yield, precision and accuracy. These assays gave mean accuracy values higher than 89 % with RSD values which were always less than 4.1 %. The method has a more simple operation process and achieves higher accuracy and precision than SPE. It has been successfully applied to plasma samples from rats after oral administration of target compounds.

Original language	English
Pages (from-to)	451-456
Number of pages	6
Journal	Chromatographia
Volume	79
Issue number	7-8
DOIs	https://doi.org/10.1007/s10337-016-3060-x
Publication status	Published - 2016 Apr 1
Externally published	Yes

Keywords

C
DSPE
HPLC
MAX
Rat plasma

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry
Organic Chemistry

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10.1007/s10337-016-3060-x

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title = "Simultaneous Analysis of Hydrochlorothiazide, Triamterene and Reserpine in Rat Plasma by HPLC and DSPE",

abstract = "A dispersive solid-phase extraction (DSPE) method of mixing two different materials (C18 and MAX) was developed as extraction procedure, which provided better extraction yields (>82.1 %) for all analytes and more appropriate sample purification from endogenous interference materials compared with common SPE. Analyte separation was achieved on a C18 reversed phase column at the wavelength of 265 nm by high performance liquid chromatography (HPLC). The method was validated in terms of extraction yield, precision and accuracy. These assays gave mean accuracy values higher than 89 % with RSD values which were always less than 4.1 %. The method has a more simple operation process and achieves higher accuracy and precision than SPE. It has been successfully applied to plasma samples from rats after oral administration of target compounds.",

keywords = "C, DSPE, HPLC, MAX, Rat plasma",

author = "Xiaogang Chen and Weidi Teng and Lei Miao and Yan Wu and Dazhi Chen and Liyan Huang and Jiahui Pan and Ning Wang and Jianzhang Fang and Yong Liang",

note = "Funding Information: This work was supported by Guangzhou science and technology plan projects 2014J4100007 and Foshan Chancheng Industry-Focused Research Funding 20141072018. Publisher Copyright: {\textcopyright} 2016, Springer-Verlag Berlin Heidelberg.",

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doi = "10.1007/s10337-016-3060-x",

language = "English",

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T1 - Simultaneous Analysis of Hydrochlorothiazide, Triamterene and Reserpine in Rat Plasma by HPLC and DSPE

AU - Chen, Xiaogang

AU - Teng, Weidi

AU - Miao, Lei

AU - Wu, Yan

AU - Chen, Dazhi

AU - Huang, Liyan

AU - Pan, Jiahui

AU - Wang, Ning

AU - Fang, Jianzhang

AU - Liang, Yong

N1 - Funding Information: This work was supported by Guangzhou science and technology plan projects 2014J4100007 and Foshan Chancheng Industry-Focused Research Funding 20141072018. Publisher Copyright: © 2016, Springer-Verlag Berlin Heidelberg.

PY - 2016/4/1

Y1 - 2016/4/1

N2 - A dispersive solid-phase extraction (DSPE) method of mixing two different materials (C18 and MAX) was developed as extraction procedure, which provided better extraction yields (>82.1 %) for all analytes and more appropriate sample purification from endogenous interference materials compared with common SPE. Analyte separation was achieved on a C18 reversed phase column at the wavelength of 265 nm by high performance liquid chromatography (HPLC). The method was validated in terms of extraction yield, precision and accuracy. These assays gave mean accuracy values higher than 89 % with RSD values which were always less than 4.1 %. The method has a more simple operation process and achieves higher accuracy and precision than SPE. It has been successfully applied to plasma samples from rats after oral administration of target compounds.

AB - A dispersive solid-phase extraction (DSPE) method of mixing two different materials (C18 and MAX) was developed as extraction procedure, which provided better extraction yields (>82.1 %) for all analytes and more appropriate sample purification from endogenous interference materials compared with common SPE. Analyte separation was achieved on a C18 reversed phase column at the wavelength of 265 nm by high performance liquid chromatography (HPLC). The method was validated in terms of extraction yield, precision and accuracy. These assays gave mean accuracy values higher than 89 % with RSD values which were always less than 4.1 %. The method has a more simple operation process and achieves higher accuracy and precision than SPE. It has been successfully applied to plasma samples from rats after oral administration of target compounds.

KW - C

KW - DSPE

KW - HPLC

KW - MAX

KW - Rat plasma

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DO - 10.1007/s10337-016-3060-x

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SN - 0009-5893

VL - 79

SP - 451

EP - 456

JO - Chromatographia

JF - Chromatographia

IS - 7-8

ER -

Simultaneous Analysis of Hydrochlorothiazide, Triamterene and Reserpine in Rat Plasma by HPLC and DSPE

Abstract

Keywords

ASJC Scopus subject areas

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