バイオ蛍光式アセトンガス動画像化カメラのための酵素固定化メッシュの作製と評価

Kenta Iitani; Mika Suzuki; Koji Toma; Takahiro Arakawa; Kohji Mitsubayashi

doi:10.1541/ieejsmas.142.166

バイオ蛍光式アセトンガス動画像化カメラのための酵素固定化メッシュの作製と評価

Translated title of the contribution: An Enzyme Immobilized Mesh for Bio-fluorometric Acetone Gas-imaging Camera

Kenta Iitani, Mika Suzuki, Koji Toma, Takahiro Arakawa, Kohji Mitsubayashi

Research output: Contribution to journal › Article › peer-review

Abstract

Human-derived acetone vapor could be utilized in clinical practice. In this paper, we prepared and demonstrated secondary alcohol dehydrogenase (S-ADH) immobilized mesh toward acetone vapor imaging. Immobilized S-ADH activity was evaluated by autofluorescence of reduced nicotinamide adenine dinucleotide which is consumed in S-ADH catalytic reaction with acetone. As a result, a glutaraldehyde crosslink method showed higher S-ADH activity and allowed fluorometric imaging of acetone vapor without distortions.

Translated title of the contribution	An Enzyme Immobilized Mesh for Bio-fluorometric Acetone Gas-imaging Camera
Original language	Japanese
Pages (from-to)	166-167
Number of pages	2
Journal	IEEJ Transactions on Sensors and Micromachines
Volume	142
Issue number	7
DOIs	https://doi.org/10.1541/ieejsmas.142.166
Publication status	Published - 2022
Externally published	Yes

ASJC Scopus subject areas

Mechanical Engineering
Electrical and Electronic Engineering

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10.1541/ieejsmas.142.166

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title = "バイオ蛍光式アセトンガス動画像化カメラのための酵素固定化メッシュの作製と評価",

abstract = "Human-derived acetone vapor could be utilized in clinical practice. In this paper, we prepared and demonstrated secondary alcohol dehydrogenase (S-ADH) immobilized mesh toward acetone vapor imaging. Immobilized S-ADH activity was evaluated by autofluorescence of reduced nicotinamide adenine dinucleotide which is consumed in S-ADH catalytic reaction with acetone. As a result, a glutaraldehyde crosslink method showed higher S-ADH activity and allowed fluorometric imaging of acetone vapor without distortions.",

keywords = "acetone, fluorescence imaging, gas-phase biosensor, metabolism assessment, secondary alcohol dehydrogenase",

author = "Kenta Iitani and Mika Suzuki and Koji Toma and Takahiro Arakawa and Kohji Mitsubayashi",

note = "Publisher Copyright: {\textcopyright} 2022 The Institute of Electrical Engineers of Japan.",

year = "2022",

doi = "10.1541/ieejsmas.142.166",

language = "Japanese",

volume = "142",

pages = "166--167",

journal = "IEEJ Transactions on Sensors and Micromachines",

issn = "1341-8939",

publisher = "The Institute of Electrical Engineers of Japan",

number = "7",

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TY - JOUR

T1 - バイオ蛍光式アセトンガス動画像化カメラのための酵素固定化メッシュの作製と評価

AU - Iitani, Kenta

AU - Suzuki, Mika

AU - Toma, Koji

AU - Arakawa, Takahiro

AU - Mitsubayashi, Kohji

PY - 2022

Y1 - 2022

N2 - Human-derived acetone vapor could be utilized in clinical practice. In this paper, we prepared and demonstrated secondary alcohol dehydrogenase (S-ADH) immobilized mesh toward acetone vapor imaging. Immobilized S-ADH activity was evaluated by autofluorescence of reduced nicotinamide adenine dinucleotide which is consumed in S-ADH catalytic reaction with acetone. As a result, a glutaraldehyde crosslink method showed higher S-ADH activity and allowed fluorometric imaging of acetone vapor without distortions.

AB - Human-derived acetone vapor could be utilized in clinical practice. In this paper, we prepared and demonstrated secondary alcohol dehydrogenase (S-ADH) immobilized mesh toward acetone vapor imaging. Immobilized S-ADH activity was evaluated by autofluorescence of reduced nicotinamide adenine dinucleotide which is consumed in S-ADH catalytic reaction with acetone. As a result, a glutaraldehyde crosslink method showed higher S-ADH activity and allowed fluorometric imaging of acetone vapor without distortions.

KW - acetone

KW - fluorescence imaging

KW - gas-phase biosensor

KW - metabolism assessment

KW - secondary alcohol dehydrogenase

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EP - 167

JO - IEEJ Transactions on Sensors and Micromachines

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バイオ蛍光式アセトンガス動画像化カメラのための酵素固定化メッシュの作製と評価

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