A high-throughput screening utilization intramolecular fluorescence resonance energy transfer for the discovery of the molecules that bind HIV-1 TAR RNA specifically

Chiharu Matsumoto; Keita Hamasaki; Hisakazu Mihara; Akihiko Ueno

doi:10.1016/S0960-894X(00)00359-0

A high-throughput screening utilization intramolecular fluorescence resonance energy transfer for the discovery of the molecules that bind HIV-1 TAR RNA specifically

Chiharu Matsumoto, Keita Hamasaki, Hisakazu Mihara, Akihiko Ueno

研究成果: Article › 査読

57 被引用数 (Scopus)

抄録

A 16-residue peptide, including the Tat_49-57 sequence was labeled with a fluorescein and a tetramethylrhodamine at its N- and C-terminus, respectively. This double dye-labeled peptide was prepared as a tracer for high-throughput screening utilizing intramolecular fluorescence resonance energy transfer (FRET). The binding of the competitor molecules for HIV-1 TAR RNA were monitored and dissociation constants of those molecule were determined by using this tracer. This novel screening system might be useful to discover the drug for HIV-1 TAR RNA. (C) 2000 Elsevier Science Ltd. All rights reserved.

本文言語	English
ページ（範囲）	1857-1861
ページ数	5
ジャーナル	Bioorganic and Medicinal Chemistry Letters
巻	10
号	16
DOI	https://doi.org/10.1016/S0960-894X(00)00359-0
出版ステータス	Published - 2000 8月 21
外部発表	はい

ASJC Scopus subject areas

生化学
分子医療
分子生物学
薬科学
創薬
臨床生化学
有機化学

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10.1016/S0960-894X(00)00359-0

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引用スタイル

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