TY - JOUR
T1 - A Klebsiella aerogenes moaEF operon is controlled by the positive MoaR regulator of the monoamine regulon
AU - Azakami, Hiroyuki
AU - Sugino, Hiroyuki
AU - Iwata, Nobuhide
AU - Yokoro, Natsuko
AU - Yamashita, Mitsuo
AU - Murooka, Yoshikatsu
PY - 1995/10/16
Y1 - 1995/10/16
N2 - A 30-kDa protein accumulated upon induction by a high concentration of tyramine or dopamine in cells of Klebsiella aerogenes (Ka). These cells carried a plasmid (pAS123) that included the arylsulfatase operon (atsBA). Deletion analysis showed that the region essential for induction of the 30-kDa protein was located within a 2.0-kb cloned segment downstream of the atsBA operon. The nucleotide (nt) sequence of the 2.0-kb fragment revealed two open reading frames (ORFs), moaE and moaF. Transcription from a putative promoter of moaE was induced by the addition of tyramine, and the moaF gene was co-transcribed from this monoamine-inducible Ka promoter. The deduced Ka MoaE protein was homologous to insect-type alcohol dehydrogenase. The sequence of the 18 amino acids from the N-terminus of the purified 30-kDa protein agreed with that deduced from the nt sequence of moaF. Using a Ka strain with a mutant moaR gene, we found that MoaR, that acts as the positive regulator of the monoamine regulon, also acts as the positive regulator of the moaEF operon.
AB - A 30-kDa protein accumulated upon induction by a high concentration of tyramine or dopamine in cells of Klebsiella aerogenes (Ka). These cells carried a plasmid (pAS123) that included the arylsulfatase operon (atsBA). Deletion analysis showed that the region essential for induction of the 30-kDa protein was located within a 2.0-kb cloned segment downstream of the atsBA operon. The nucleotide (nt) sequence of the 2.0-kb fragment revealed two open reading frames (ORFs), moaE and moaF. Transcription from a putative promoter of moaE was induced by the addition of tyramine, and the moaF gene was co-transcribed from this monoamine-inducible Ka promoter. The deduced Ka MoaE protein was homologous to insect-type alcohol dehydrogenase. The sequence of the 18 amino acids from the N-terminus of the purified 30-kDa protein agreed with that deduced from the nt sequence of moaF. Using a Ka strain with a mutant moaR gene, we found that MoaR, that acts as the positive regulator of the monoamine regulon, also acts as the positive regulator of the moaEF operon.
KW - DNA sequence
KW - Tyramine
KW - catecholamine metabolism
KW - dopamine
KW - transcriptional regulation
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U2 - 10.1016/0378-1119(95)00400-Z
DO - 10.1016/0378-1119(95)00400-Z
M3 - Article
C2 - 7590328
AN - SCOPUS:0028889837
SN - 0378-1119
VL - 164
SP - 89
EP - 94
JO - Gene
JF - Gene
IS - 1
ER -