TY - CONF
T1 - A photoresponsive polymer surface which enables on-demand manipulation of adhesive cells
AU - Edahiro, Jun Ichi
AU - Sumaru, Kimio
AU - Tada, Yuichi
AU - Takagi, Toshiyuki
AU - Shinbo, Toshio
AU - Kanamori, Toshiyuki
AU - Yoshimi, Yasuo
PY - 2005/12/1
Y1 - 2005/12/1
N2 - We have been developing a novel "on-demand cell manipulation technique" which enables control of cell adhesion by regional photo-irradiation on adhering region of objective cells. In this study, our objective is to improve performance of the "photoresponsive culture surface (PRCS) " that can control cell adhesion by photo-irradiation and temperature change and that is the key material for our novel technique. Firstly, cell adhesion control of extended cells was successfully achieved for the first time. PRCS was prepared with pory(N-isopropylacrylamide) containing photochromic chromophore, 6-nitrospiropyran. On this PRCS, MDCK cells were attached and extended evenly as a sheet after incubation at 37°C. The PRCS was regionally irradiated with light (365 nm) and cooled at 15°C. Then the PRCS was washed by fresh medium to remove weakly adhering cells in non-irradiated region. As a result, we confirmed that this method was applicable even to extended cells. Additionally, co-culture system was produced in simple procedures by culturing another kind of cells (CHO-K1 cells) in the cell-detached area. Secondary, for lowering cell damage from low temperature treatment, we prepared another type of PRCS with poly(N,N-dimethylacrylamide) containing 6-nitrospiropyran, which has no thermoresponsive nature. We confirmed that adhesion of MDCK cells on this novel PRCS could be changed only by photo-irradiation without any temperature change. Additionally, this result also supports our hypothesis that spiropyran chromophore directly captures cells in cell adhesion control.
AB - We have been developing a novel "on-demand cell manipulation technique" which enables control of cell adhesion by regional photo-irradiation on adhering region of objective cells. In this study, our objective is to improve performance of the "photoresponsive culture surface (PRCS) " that can control cell adhesion by photo-irradiation and temperature change and that is the key material for our novel technique. Firstly, cell adhesion control of extended cells was successfully achieved for the first time. PRCS was prepared with pory(N-isopropylacrylamide) containing photochromic chromophore, 6-nitrospiropyran. On this PRCS, MDCK cells were attached and extended evenly as a sheet after incubation at 37°C. The PRCS was regionally irradiated with light (365 nm) and cooled at 15°C. Then the PRCS was washed by fresh medium to remove weakly adhering cells in non-irradiated region. As a result, we confirmed that this method was applicable even to extended cells. Additionally, co-culture system was produced in simple procedures by culturing another kind of cells (CHO-K1 cells) in the cell-detached area. Secondary, for lowering cell damage from low temperature treatment, we prepared another type of PRCS with poly(N,N-dimethylacrylamide) containing 6-nitrospiropyran, which has no thermoresponsive nature. We confirmed that adhesion of MDCK cells on this novel PRCS could be changed only by photo-irradiation without any temperature change. Additionally, this result also supports our hypothesis that spiropyran chromophore directly captures cells in cell adhesion control.
KW - Cell adhesion control
KW - On-demand cell manipulation
KW - Photoresponsive culture surface
KW - Photoresponsive polymer
KW - Spiropyran. N-isopropylacrylamide
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UR - http://www.scopus.com/inward/citedby.url?scp=33645569848&partnerID=8YFLogxK
M3 - Paper
AN - SCOPUS:33645569848
SP - 5213
EP - 5214
T2 - 54th SPSJ Symposium on Macromolecules
Y2 - 20 September 2005 through 22 September 2005
ER -