TY - GEN
T1 - All-in-one uniified microfluidic chip for automation of embryonic cell manipulation
AU - Sakuma, Shinya
AU - Yamanishi, Yoko
AU - Arai, Fumihito
AU - Arai, Tatsuo
AU - Hasegawa, Akiyuki
AU - Tanikawa, Tamio
AU - Ichikawa, Akihiko
AU - Satoh, Osamu
AU - Nakayama, Akihiro
AU - Aso, Hiroshi
AU - Goto, Mitsuhiro
AU - Takahashi, Seiya
AU - Matsukawa, Kazutsugu
PY - 2009
Y1 - 2009
N2 - We developed a microfluidic chip for automation of cloning process based on a new protocol. The protocol is based on removal of the zona pellucida outside the chip which contributes to simplify on-chip automation of cloning. Then, the oocytes are put into the chip. The design concept of the chip is summarized as follows. (1) The oocyte is cut into two parts. (2) The divided half oocyte is sorted with and without nucleus. (3) The half oocyte without nucleus is coupled with a donor cell, and they are fused by an electrical field. For the current study, the microfluidic chip was designed to have 3 parts for this process. Then, all-in-one unified microfluidic chip was successfully fabricated. Basic functions of these parts are verified independently.
AB - We developed a microfluidic chip for automation of cloning process based on a new protocol. The protocol is based on removal of the zona pellucida outside the chip which contributes to simplify on-chip automation of cloning. Then, the oocytes are put into the chip. The design concept of the chip is summarized as follows. (1) The oocyte is cut into two parts. (2) The divided half oocyte is sorted with and without nucleus. (3) The half oocyte without nucleus is coupled with a donor cell, and they are fused by an electrical field. For the current study, the microfluidic chip was designed to have 3 parts for this process. Then, all-in-one unified microfluidic chip was successfully fabricated. Basic functions of these parts are verified independently.
KW - Cloning
KW - Non-contact manipulation
KW - μ-TAS
UR - http://www.scopus.com/inward/record.url?scp=84884397561&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884397561&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84884397561
SN - 9780979806421
T3 - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1883
EP - 1885
BT - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Y2 - 1 November 2009 through 5 November 2009
ER -