Blood heparin sensor made from a paste electrode of graphite particles grafted with molecularly imprinted polymer

Yasuo Yoshimi, Yuto Yagisawa, Rina Yamaguchi, Maki Seki

研究成果: Article査読

25 被引用数 (Scopus)


A real-time heparin monitor could be used to optimize the dosage of heparin during extracorporeal circulation procedures. This report describes the development of a graphite-paste (GP) electrode with molecularly imprinted polymer (MIP) grafted onto it. Heparin-imprinted poly (methacryloxyethyltriammonium chloride -co- acrylamide -co- methylenebisacrylamide) was grafted directly onto graphite particles. The grafted particles were thoroughly mixed with oil to fabricate the MIP-GP electrode. Traditional cyclic voltammetry was performed with the electrode in physiological saline or bovine whole blood containing 5 mM ferrocyanide and 0–8 units/mL heparin. The current intensity increased with heparin concentration, due to expansion of the effective surface area resulting from heparin-promoted mobility of the oil in the MIP-GP electrode. No significant difference was found in the sensitivity of the current to unfractionated heparin among the electrodes fabricated because of the electrode homogenization resulting from thorough mixing of the MIP-grafted particles and oil. (A previous MIP-grafted indium tin oxide electrode exhibited lower sensitivity in blood than in saline.) Only 60 s were needed to stabilize the current. The current at the MIP-GP electrode was also sensitive to low-molecular-weight heparin in blood, but insensitive to chondroitin sulfate C (CSC), which is a heparin analog. The non-imprinted polymer (NIP)-grafted electrode was insensitive to heparin. Thus, the MIP-GP electrode, which operated through a new heparin-sensing mechanism, is an excellent candidate for application as a disposable sensor to monitor heparin levels in blood.

ジャーナルSensors and Actuators, B: Chemical
出版ステータスPublished - 2018 4月 15

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 器械工学
  • 凝縮系物理学
  • 表面、皮膜および薄膜
  • 金属および合金
  • 電子工学および電気工学
  • 材料化学


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