@article{64d05e19a72747d78c137ea6ff05d336,
title = "Determination of menadione by liquid chromatography-tandem mass spectrometry using pseudo multiple reaction monitoring",
abstract = "This study aimed to develop a menadione (MD) determination method employing liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a pseudo multiple reaction monitoring (MRM) technique, wherein two quadrupoles are used to monitor the same ion. Detection limits of 40 and 2 pg were obtained for MD and its deuterium-labeled form, respectively, whereas MD intra- and inter-assay coefficient of variation values were determined as 5.4 - 8.2%, with the corresponding recoveries equaling 90.5 - 109.6%. The developed method enables determination of MD in urine, plasma, cell extract, and culture media, demonstrating that pseudo multiple reaction monitoring can achieve quantification of compounds forming no suitable product ions, such as MD.",
keywords = "LC-MS/MS, Menadione, Pseudo MRM, Vitamin K",
author = "Maya Kamao and Yoshihisa Hirota and Yoshitomo Suhara and Naoko Tsugawa and Kimie Nakagawa and Toshio Okano and Hiroshi Hasegawa",
note = "Funding Information: We thank Madoka Ogata, Mizusa Inui, Akihiro Hamana, Manaka Hayashi, Yuki Kamiya, Syunsuke Sakata, and Mari Nagao for their excellent technical assistance. This work was partly supported by a Grant-in-aid for Challenging Exploratory Research (Grant No. 26560072) from the Japan Society for the Promotion of Science (JSPS). Publisher Copyright: {\textcopyright} The Japan Society for Analytical Chemistry.",
year = "2017",
doi = "10.2116/analsci.33.863",
language = "English",
volume = "33",
pages = "863--867",
journal = "Analytical Sciences",
issn = "0910-6340",
publisher = "Japan Society for Analytical Chemistry",
number = "7",
}