抄録
High-performance liquid chromatography (HPLC) with UV detection for the simultaneous determination of the free form of p-hydroxymethamphetamine (p-OHMA) and its metabolite, glucuronide (p-OHMAG) was accomplished for the first time. We achieved this by employing 1) an ion pair reagent for retention of sample to a solid-phase extraction (SPE) cartridge, Sep-Pak® Light C18 and 2) a simple two-step stepwise elution technique for subsequent ion pair RP-HPLC. The proposed method was optimized for resolution of p-OHMAG, p-OHMA and MA. The method was successfully applied to urine samples collected from MA abusers.
| 本文言語 | English |
|---|---|
| ページ（範囲） | 493-495 |
| ページ数 | 3 |
| ジャーナル | Chemical and Pharmaceutical Bulletin |
| 巻 | 54 |
| 号 | 4 |
| DOI | |
| 出版ステータス | Published - 2006 4月 1 |
| 外部発表 | はい |
ASJC Scopus subject areas
- 化学 (全般)
- 創薬