TY - GEN
T1 - Electrically-induced bubble knife for protein crystallization and processing
AU - Kuriki, Hiroki
AU - Takasawa, Sou
AU - Sakuma, Shinya
AU - Kurisu, Genji
AU - Arai, Fumihito
AU - Yamanishi, Yoko
PY - 2013/1/1
Y1 - 2013/1/1
N2 - We have succeeded in protein crystallization and processing by electrically-induced bubble knife. The dispensed directional mono-dispersed micro-bubbles whose air-liquid interface tends to combine the ambient molecules due to by their stiction force on the surface of micro-bubbles. Electrically-induced bubble knife enables to generate mono-dispersed micro-bubbles by the electrolysis and local heat, and their generation speed was 30.8 kHz. These characteristics enable to agglomerate the protein molecule which brings to successful protein crystallization in the solution with low concentration. The rate of protein crystal-nucleation by electrically-induced mono-dispersed micro-bubble was much higher than that of control condition. Furthermore, it was confirmed that cavitation induced by electrically-induced bubble knife enabled to curve protein crystal. It seemed that it has potential to contribute to more refined analysis.
AB - We have succeeded in protein crystallization and processing by electrically-induced bubble knife. The dispensed directional mono-dispersed micro-bubbles whose air-liquid interface tends to combine the ambient molecules due to by their stiction force on the surface of micro-bubbles. Electrically-induced bubble knife enables to generate mono-dispersed micro-bubbles by the electrolysis and local heat, and their generation speed was 30.8 kHz. These characteristics enable to agglomerate the protein molecule which brings to successful protein crystallization in the solution with low concentration. The rate of protein crystal-nucleation by electrically-induced mono-dispersed micro-bubble was much higher than that of control condition. Furthermore, it was confirmed that cavitation induced by electrically-induced bubble knife enabled to curve protein crystal. It seemed that it has potential to contribute to more refined analysis.
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U2 - 10.1109/MHS.2013.6710474
DO - 10.1109/MHS.2013.6710474
M3 - Conference contribution
AN - SCOPUS:84893797876
SN - 9781479915293
T3 - 2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013
BT - 2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013
PB - IEEE Computer Society
T2 - 2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013
Y2 - 10 November 2013 through 13 November 2013
ER -