Fabrication of a flow-type micro cell chip based on photo-induced cell capturing

By using a photo-responsive cell culture surface, which captures the cell strongly in response to light irradiation, we examined a novel layout of micro cell chip, in which living cells were immobilized in a prescribed arrangement in micro channels. We introduced a cell suspension into the micro channel, irradiated the near-UV light locally and washed with buffer solution. As a result of repeating these operation using two different kinds of cells, it was confirmed that the living cells of different kinds were placed in the prescribed positions in the micro channel without damaging their viability.