Fundamental study for detection of anisakis by photoacoustic imaging

Nao Yoshida, Takeshi Namita, Kengo Kondo, Makoto Yamakawa, Tsuyoshi Shiina

研究成果: Conference contribution


Anisakis larvae infest fish of several types. Consumption of raw fish products containing anisakis can engender a severe human disease known as anisakidosis. Therefore, anisakis must be removed from fish before consumption. Conventional screening methods such as candling can be done easily to detect surface parasites, but such methods are not accurate or sufficiently sensitive to detect parasites that are embedded deeply in fish muscle. To resolve these shortcomings, we devised a method using photoacoustic imaging. This imaging modality combines high tissue specificity with better penetration depth. As a fundamental study, photoacoustic spectra of anisakis and mackerel were measured. Silica glass cells containing a sample were irradiated with nanosecond pulses of laser light (300-700 nm wavelengths). Photoacoustic signals were measured using a hydrophone. The beam sampler reflected part of the incident light. Then the light intensity was measured. The photoacoustic signal intensity was normalized. The spectrum of anisakis decreases monotonically. The spectrum of mackerel increases moderately in 375-400 nm and 490-540 nm. To examine the ability to distinguish between anisakis and mackerel using photoacoustic spectrum differences, the intensity ratio at photoacoustic signals of 375 nm and 400 nm (I375 nm / I400 nm) and 490 nm and 530 nm (I490 nm / I530 nm) was calculated. The ratio of anisakis is higher than 1.0, although the ratio found for mackerel is less than 1.0. These analyses demonstrated the possibility of detecting anisakis in mackerel using multi-wavelength analysis.

ホスト出版物のタイトルPhotons Plus Ultrasound
ホスト出版物のサブタイトルImaging and Sensing 2021
編集者Alexander A. Oraevsky, Lihong V. Wang
出版ステータスPublished - 2021
イベントPhotons Plus Ultrasound: Imaging and Sensing 2021 - Virtual, Online, United States
継続期間: 2021 3月 62021 3月 11


名前Progress in Biomedical Optics and Imaging - Proceedings of SPIE


ConferencePhotons Plus Ultrasound: Imaging and Sensing 2021
国/地域United States
CityVirtual, Online

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 原子分子物理学および光学
  • 生体材料
  • 放射線学、核医学およびイメージング


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