TY - GEN
T1 - High-speed single cell dispensing system
AU - Kawahara, Tomohiro
AU - Hirano, Tatsuhiko
AU - Feng, Lin
AU - Uvet, Huseyin
AU - Hagiwara, Masaya
AU - Yamanishi, Yoko
AU - Arai, Fumihito
PY - 2011
Y1 - 2011
N2 - In this paper, we discuss the single cell dispensing system to increase both the speed and the success rate of single cell dispensing. Two pairs of capacitance sensors are placed in a biochip to detect the flow velocity of cells, and the air pressure is applied to eject cells by synchronizing the timing. Then, the system theoretically has a capability to eject 3 cells/sec with maximum flow velocity is 10 mm/sec. Finally, we succeeded in automatic dispensing of a single polystyrene bead (=100 μm) from a biochip to culture well atmosphere using developed cell ejection system with the success rate of 50 %. Furthermore, we also succeeded in single swine oocyte dispensing by developed system.
AB - In this paper, we discuss the single cell dispensing system to increase both the speed and the success rate of single cell dispensing. Two pairs of capacitance sensors are placed in a biochip to detect the flow velocity of cells, and the air pressure is applied to eject cells by synchronizing the timing. Then, the system theoretically has a capability to eject 3 cells/sec with maximum flow velocity is 10 mm/sec. Finally, we succeeded in automatic dispensing of a single polystyrene bead (=100 μm) from a biochip to culture well atmosphere using developed cell ejection system with the success rate of 50 %. Furthermore, we also succeeded in single swine oocyte dispensing by developed system.
UR - http://www.scopus.com/inward/record.url?scp=84863315376&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84863315376&partnerID=8YFLogxK
U2 - 10.1109/MHS.2011.6102235
DO - 10.1109/MHS.2011.6102235
M3 - Conference contribution
AN - SCOPUS:84863315376
SN - 9781457713613
T3 - 2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"
SP - 472
EP - 474
BT - 2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"
PB - IEEE Computer Society
T2 - 22nd Annual Symp. on Micro-Nano Mechatronics and Human Science, MHS 2011, Held Jointly with the Symp. on COE for Education and Research of Micro-Nano Mechatronics, Micro-Nano GCOE 2011, Symp. on Hyper Bio Assembler for 3D Cellular System Innovation
Y2 - 6 November 2011 through 9 November 2011
ER -