TY - GEN
T1 - Micro ELISA chip to Detect Multiple Allergic Proteins Simultaneously
AU - Abe, Yuki
AU - Hasegawa, Tadahiro
AU - Yamasita, Mitsuo
N1 - Publisher Copyright:
© 2019 IEEE.
PY - 2019/4/25
Y1 - 2019/4/25
N2 - The ELISA (Enzyme-Linked Immuno Sorbent Assay) chip to detect multiple allergic proteins simultaneously was developed successfully. This chip consists of multiple nitrocellulose membrane with 1mm diameter that immobilized several kinds of allergic proteins with 50ng amount only is fabricated using the multi-layer lithography. The experimental results demonstrated that 1) the specific allergen protein, and 2) the quantitative change of the antigen-antibody reaction depending on amount of antibody were able to be detected without contamination. The detection procedure that is only to inject antibodies in order into the chip is simple. The time for the whole detection procedure is short with 16 min. This ELISA chip that can specify the allergen protein that causes the patient's allergic disease is useful for the hyposensitization therapy.
AB - The ELISA (Enzyme-Linked Immuno Sorbent Assay) chip to detect multiple allergic proteins simultaneously was developed successfully. This chip consists of multiple nitrocellulose membrane with 1mm diameter that immobilized several kinds of allergic proteins with 50ng amount only is fabricated using the multi-layer lithography. The experimental results demonstrated that 1) the specific allergen protein, and 2) the quantitative change of the antigen-antibody reaction depending on amount of antibody were able to be detected without contamination. The detection procedure that is only to inject antibodies in order into the chip is simple. The time for the whole detection procedure is short with 16 min. This ELISA chip that can specify the allergen protein that causes the patient's allergic disease is useful for the hyposensitization therapy.
UR - http://www.scopus.com/inward/record.url?scp=85065649286&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85065649286&partnerID=8YFLogxK
U2 - 10.1109/SII.2019.8700438
DO - 10.1109/SII.2019.8700438
M3 - Conference contribution
AN - SCOPUS:85065649286
T3 - Proceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019
SP - 675
EP - 678
BT - Proceedings of the 2019 IEEE/SICE International Symposium on System Integration, SII 2019
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2019 IEEE/SICE International Symposium on System Integration, SII 2019
Y2 - 14 January 2019 through 16 January 2019
ER -