TY - CONF
T1 - Photo-control of cell adhesion in a flow-type cell chip
AU - Tada, Yuichi
AU - Sumaru, Kimio
AU - Sugiura, Shinji
AU - Edahiro, Junichi
AU - Takagi, Toshiyuki
AU - Shinbo, Toshio
AU - Kanamori, Toshiyuki
AU - Yoshimi, Yasuo
PY - 2005/12/1
Y1 - 2005/12/1
N2 - We developed a novel cell chip, which enables us to control cell adhesion of the bottom face in a micro channel by local light irradiation. A flow-type cell chip was constructed by attaching a sheet of poly (dimethyl siloxane) having micro-channel structure at surface, to a photo-responsive cell culture surface, which was functionalized with poly(N-isopropylacrylamide) containing nitrospiropyran residues, by pressure bonding. After systematic examination of the chip design and cell culture condition, we specified the condition allowing to culture living cells in the micro channel for more than 30 hours. We also confirmed that the irradiation of UV light with the wavelength of 365 nm brought a drastic enhancement of cell adhesion as we confirmed previously in dish-like culture system even in a micro channel of the flow-type chip. Practically we succeeded to introduce living cell only in a required region of a micro channel, and to remove the cells from the other region (Fig.1). Further, by using a fluorescent dye, which stains living cells only, viability of the cells was confirmed to be maintained well even after the procedure of cell adhesion control.
AB - We developed a novel cell chip, which enables us to control cell adhesion of the bottom face in a micro channel by local light irradiation. A flow-type cell chip was constructed by attaching a sheet of poly (dimethyl siloxane) having micro-channel structure at surface, to a photo-responsive cell culture surface, which was functionalized with poly(N-isopropylacrylamide) containing nitrospiropyran residues, by pressure bonding. After systematic examination of the chip design and cell culture condition, we specified the condition allowing to culture living cells in the micro channel for more than 30 hours. We also confirmed that the irradiation of UV light with the wavelength of 365 nm brought a drastic enhancement of cell adhesion as we confirmed previously in dish-like culture system even in a micro channel of the flow-type chip. Practically we succeeded to introduce living cell only in a required region of a micro channel, and to remove the cells from the other region (Fig.1). Further, by using a fluorescent dye, which stains living cells only, viability of the cells was confirmed to be maintained well even after the procedure of cell adhesion control.
KW - Cell adhesion
KW - Cell patterning
KW - Micro channel
KW - Photo-responsive cell culture surface
KW - Photo-responsive polymer
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M3 - Paper
AN - SCOPUS:33645651799
T2 - 54th SPSJ Symposium on Macromolecules
Y2 - 20 September 2005 through 22 September 2005
ER -